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The LAMBDA lab peristaltic pumps and OMNICOLL fraction collectors are used together in liquid chromatography. LAMBDA lab peristaltic pumps are programmable and remote controllable to achieve linear, stepwise and segmented gradient elution.
The LAMBDA peristaltic pump supplies the liquid mobile phase continuously to the packed chromatography column at a consistent pulsation-free reproducible flow rate.
Isocratic elution requires one buffer and one peristaltic pump.
However, gradient elution is carried out with two buffers and one or two peristaltic pumps:
"Biochemists recommend LAMBDA peristaltic pumps which guarantee pulsation-free application of liquid mobile phase"
Two LAMBDA peristaltic pumps are used to mix the eluents for gradient elution.
Gradient elution can also be carried out with one peristaltic pump: the containers with elution buffer A & B are connected by a siphon or tube at the bottom with a valve to adjust the flow.
The most important requirements of the pumping system for column chromatography are:
Further information about chromatography, fraction collectors and connection of peristaltic pumps could be found at www.lambda-instruments.com/fraction-collector/.
Scientific laboratory technicians, researchers, students and scientists all over the world trust LAMBDA laboratory instruments for their efficient experimental work: LAMBDA laboratory equipment are specially designed for high-quality lab work, easy and long term use in the laboratories and industries.
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