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Biochemical characterization of GAPORMm produced in MINIFOR fermenter
Enzyme characterization studies are important to obtain valuable information that can help future studies in molecular engineering, proteomics, metabolomics, among other biotechnological applications. On this field, Japanese researchers from the R & D Division of Fujirebio, Inc. investigated, for the first time, the enzyme GAPOR from M. maripaludis to understand its biochemical and biophysical characteristics.
In this experiment, the researchers cultivated M. maripaludis and genomic DNA of GAPOR was extracted from a recombinant E. Coli grown in the LAMBDA MINIFOR fermenter system to obtain GAPORMm for the biochemical and biophysical characterization.
Figure 1. GAPORMm expressed in E. coli using MINIFOR fermenter. [https://doi.org/10.1128/JB.00828-07]
Transcript and enzyme assays showed that GAPORMm only operates in the glycolytic direction and it is more likely to play a metabolic role under non-steady-state growth conditions (for example, stationary phase or stress) or under growth conditions dependent on alternative substrates.
Anaerobic fermentation requires a nitrogen, carbon dioxide, or another by-product of the fermentation process to maintain the oxygen-free conditions. Thanks to the in-built thermal mass flow in the MINIFOR fermenter the regulation of the nitrogen flow can be achieved.
Among various advantages of MINIFOR we can highlight:
Further information about the LAMBDA MINIFOR fermenter-bioreactor, you will find on www.fermentor.net/features and www.lambda-instruments.com/fermenter-bioreactor/innovations-of-minifor-fermentor-bioreactor/
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Keywords: Methanococcus maripaludis, GAPOR, enzyme characterization, anaerobic culture, MINIFOR fermenter.
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