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LAMBDA MINIFOR Benchtop Bioreactor used for the Lab-Scale Production of Biosurfactant Rhamnolipids secreted by Pseudomonas aeruginosa A3
Biosurfactants (microbial-produced surfactants) are a promising alternative for chemically-synthesized surfactants in industrial applications. They are more effective, selective, environmentally friendly, and stable than many synthetic surfactants. To produce a large quantity of biosurfactant-rhamnolipid (RL), researchers from the Baghdad University used wild strains of Pseudomonas aeruginosa isolated from the local region of Iran. Isolated strains were cultivated in the Batch mode using LAMBDA MINIFOR 7L fermentor for the production of RL.
Reference: Faqri, A.M.A ; Hayder, N.H & Hashim, A. J. Lab-scale production of Rhamnolipid by Pseudomonas aeruginosa A3 and study its synergistic effect with certain antibiotics against some pathogenic bacteria. Iraqi Journal of Agricultural Sciences –2019:50(5):1290-1301. https://doi.org/10.13140/RG.2.2.10802.35520
After 120 h, the surface tension of the solution reached 27.2 mN/m, with 67% of emulsification activity and biomass 2.7 g/l. The RL concentration was 16 g/l. Using Silica-gel chromatography, Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectroscopy (FTIR) for spectral analysis, the biosurfactant mixture obtained was characterized mainly of both mono- and di-rhamnolipids. Cytotoxicity assays of mono-RL were done, obtaining a Minimum Inhibitory Concentration (MIC) of 250 µg/mL for most of bacterial used in the experiments, revealing a synergistic effect with antibiotics such as: ampicillin against Staphylococcus aureus and Serratia marcescens, with cefotaxime against Escherichia coli and Klebsiella pneumoniae, same as with tetracycline.
Features of LAMBDA MINIFOR bench-top fermenter could be found at www.fermentor.net/features and www.lambda-instruments.com/fermenter-bioreactor/
Keywords: Batch fermentation, MINIFOR bioreactor, biosurfactants, rhamnolipids, citotoxicity, antibiotic synergy, MIC: minimum inhibitory concentration, Pseudomonas.
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