The most innovative laboratory Bioreactor - Fermentor

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Easy Sterility ConceptExtremely compact systemOptimized vessel designElimination of head-plates

And that is not all. Check the additional innovative features of the lab-scale bioreactors.

Short description

Laboratory Fermentor-Bioreactor LAMBDA Minifor

The LAMBDA MINIFOR bench-top laboratory fermenter and bioreactor was developed as a result of the need to construct a small laboratory fermentor for volumes from 35 ml to over 6 liters. Based on long personal practical experience of fermentations we wanted to create a fermentor, which was easy to use and with the capacity to measure and control all the important parameters of the biological culture.

The fermentor had to take up minimum space on the bench but with good access to all parts. Several fermentors should when placed side by side be suitable for the optimization of the parameters of growth of culture or optimization of bio-transformations etc.

Each fermentor should be able to work independently or be connected to a PC for advanced regulation and extensive data treatment.

To keep the cost of the MINIFOR fermentor-bioreactor low, without compromising quality, several new ideas and innovations have been introduced:

• Instead of a fermentor flask with a stainless steel cover, which is expensive, we use whole glass vessels with threaded fittings. They have been used for many years in cell culture and are proved to maintain perfect sterility. Thanks to this concept, the MINIFOR fermenter-bioreactor is set up in shortest possible time.

• Instead of a traditional propeller agitator, which requires an expensive motor and magnetic coupling, we have introduced a new up-and-down agitation. A motor together with an inexpensive membrane perfectly assure sterility and produce an efficient mixing without formation of a vortex (no baffles needed). At the same time this type of mixing is gentler on cells and produces less foam. Novel biomimicking “fish-tail” stirring discs offer maximum mixing efficiency without cutting edges.

• The culture is heated by heat radiation produced in a parabolic radiator with a gold reflector placed under the fermentation vessel. The heat is absorbed gently in the culture similarly to the sun heating water. There is no overheating of the culture at any volume and expensive double-wall vessels with thermostatic baths are eliminated. At the same time tubing and cables disappear making the fermentor less complex.

• As far as possible expensive pieces of equipment have been replaced by new high performance plastics.

• By using modern microprocessors it has been possible to place all the electronics in the front part of the instrument. This makes the fermentor unbelievably compact and eliminates the casing tower. Despite its small size six parameters are measured and controlled in the basic configuration of the MINIFOR.

LAMBDA MINIFOR fermentor-bioreactor system includes these kits:

Technical descriptionAdvanced KitStart-up Kit
Reactor scaleLaboratory bench-top fermentor and bioreactor
Reactor designStirred Tank
Operation modebatch, fed-batch*, continuous*, (perfusion*)
Parameter controlstirrer speed, temperature, pH, Dissolved Oxygen, airflow, free parameter, level control for continuous mode.
* Optional Parameter control *weight control for continuous mode, antifoam, REDOX, LUMO (light)
Microprocessor With internal software, display and keypadyesyes
Agitation Automatic stirrer controller 0, 0.1, 0.2, …., 19.9, 20 Hz min. 0 Hz ( = 0 rpm) max. 20 Hz ( = 1200 rpm)yesyes
Measurement whole range 0 - 20 Hzyesyes
Temperature Automatic temperature control from 5 °C over RT to 70 °Cyesyes
Measurement with Pt100 from 0 to 99.9 °C in 0.1 °C stepsyesyes
IR (infrared) heateryesyes
Cooling loopyesyes
pH Automatic pH controller 0 - 13yesyes
Measurement with pH probe 0 -13yesyes
Acid pump (PRECIFLOW)yesoptional
Base pump (PRECIFLOW)yesoptional
Holders and rods for the pumpsyesoptional
Storage vessels and lines for acid and for baseyesoptional
DO (pO2) Automatic DO controller (proportional) 0 - 25 mg Oxygen/L (in 0.1 mg steps)yesyes
Measurement with DO probe 0 - 25 mg Oxygen/L (in 0.1 mg steps)yesoptional
Airflow Automatic aeration controller (proportional) 0 - 5 L/min in 0.01 L/min stepsyesyes
Measurement with internal Massflow 0 - 5 L/min in 0.01 L/min stepsyesyes
Free Parameter Automatic controller, socket, displayyesyes
AlertsFor high and low values of each parameteryesyes
Overpressure valveyesyes
Sampling deviceyesoptional
Cooling device for outlet gasyesyes
PC-software (FNet, SIAM), Laptopoptionaloptional
Feed and harvest pump: PRECIFLOW / MULTIFLOW / HiFLOW / MAXIFLOW / VIT-FIT / VIT-FIT HP, pump INTEGRATORSoptionaloptional
WEIGHING MODULE for continuous modeoptionaloptional
automatic Antifoam-system (ANTIFO & DOZITO)optionaloptional
O2-enrichment / 4-gas-mix: controller and massflows MASSFLOW 500 /MASSFLOW 5000optionaloptional
Air compressor AEROSILENTOoptionaloptional
REDOX probe, controller, pump / massflowoptionaloptional
PELTIER COOLER for medium / off-gasoptionaloptional
LUMO light and controller for PBRoptionaloptional
Operation manual and videoyesyes
Warranty2 years

Learn more about the technical description of LAMBDA MINIFOR

1. Controlled growth of Staphylococcus aureus under various concentrations of BAC (benzalkonium chloride) in MINIFOR fermentor:

Dana Cervinkova, Vladimir Babak, Durdica Marosevic, Iva Kubikova, and Zoran Jaglic. Microbial Drug Resistance. June 2013, 19(3): 160-167. The Role of the qacA Gene in Mediating Resistance to Quaternary Ammonium Compounds
Veterinary Research Institute, Brno, Czech Republic.
Keywords: Staphylococcus aureus, benzalkonium chloride (BAC), exponential phase, expression, real-time PCR, culture, concentration

2. MINIFOR Bioreactors for stem cells:

Shayan, N., Ebrahimi, M., Beiki, B. and Janzamin, E. 2012. A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cells. BIOTECHNOLOGY LETTERS.
Department of Regenerative Medicine, Cell Research Center, Royan Institute for Stem Cell Biology and Technology, Royan Cord Blood Bank, Tehran, Iran; Department of Stem Cells and Developmental Biology, Cell Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Keywords: Static culture; Suspension bioreactor; Umbilical cord blood; Vertical mixing

3. Bioreactors - An overview of the innovations implemented in MINIFOR bioreactors:

Lehky, P. 2003. Bioreactors - New Solutions for Old Problems. International Congress on Bioreactor Technology, Tampere, Finland.
Keywords: bioreactor, fermentor, cell culture, DO probe, gas flow-rate, gas station.

4. Anaerobic expression using the LAMBDA MINIFOR:

Park, M. -O., Mizutani, T. and Jones, P. R. 2007. Glyceraldehyde-3-Phosphate Ferredoxin Oxidoreductase from Methanococcus maripaludis. Journal of Bacteriology, Tokyo, Japan.
Fujirebio Incorporated, Japan.

Learn more about where LAMBDA MINIFOR bench-top laboratory fermenter and bioreactor was used...