Kolektor frakcí

Kolektor frakcí

Nový koncept ve sběru frakcí.

LAMBDA OMNICOLL automatický sběrač frakcí a vzorkovač

LAMBDA OMNICOLL - Automatický sběrač frakcí a vzorkovač poskytující téměř neomezenou flexibilitu - otevírá nové možnosti v oblasti chromatografie a vzorkování.

OMNICOLL lze použít společně s téměř všemi druhy nádob na vzorky a laboratorními stojánky, které jsou k dispozici na trhu. Nový koncept ve sběru frakcí. 

Neomezený rozměr:

  • mikrotitrační destičky, nádoby větších objemů, kádinky, baňky, atd.
  • neomezený počet sbíraných frakcí

Vysoce flexibilní kontrola:

  • kontrola objemu/času/počítání kapek/pomocí externího signálu
  • snadné programování a manipulace

Vícekanálový sběr:

  • jednokanálový
  • souběžný vícekanálový až do 20 frakcí

Rozšíření kapacity:

Kapacita sběrače frakcí může být navýšena přidání spodních dílů.

Typ: LAMBDA OMNICOLL – microprocessor-controlled programmable fraction collector – sampler
Režim sběru: Linear (line), meander (zigzag) or row collection
Normal modus:
Čas: 0.1 to 999.9 minutes (16.67 hours) in 0.1 min steps or 1 to 9999 minutes (166.7 hours) in 1 min steps
Objem: 0.01 to 500 ml or 0.6 to 30 litres (external counts using LAMBDA peristaltic pump)
High režim: Same as Normal modus, but with a pause between fractions (from 0.1 to 999.9 minutes or 1 to 9999 minutes)
Dálkové ovládání:
Normální režim: Collector takes a single fraction after an external voltage pulse of 3-12 V (or 12-30 V with a 3300 ohm resistor)
High režim: Collector takes a 1 to 999 fractions after a single external voltage pulse of 3-12 V (or 12-30 V with a 3300 ohm resistor)
Kapacita: Any tube rack or container type with a surface smaller than 45 x 31cm
Dodávané stojánky: 360 tubes of 12-13 mm diameter; 240 tubes of 16 mm diameter; 160 tubes of 20 mm diameter; 96 tubes of 30 mm diameter; The capacity can be increased many times by coupling several lower fraction collector parts together.
Trvalá paměť: Storage of all settings
Rozhraní: RS-232 (optional)
Napájení: 95–240 V/60–50 Hz AC plug-in power supply with DC 9V/12W output; possible field operation on 12 V accumulator
Rozměry: 34 (W) x 30 (H) x 49 (D) cm
Váha: 6.5 Kg
Bezpečnost: CE, meets IEC 1010/1 norm for laboratory instruments
Provozní teplota: 0-40 °C
Provozní vlhkost: 0-90% RH, not condensing
Dálkové ovládání: 0-10 V; (option 0-20 or 4-20 mA)

1. The elute was collected using LAMBDA OMNICOLL fraction collector in 15 mL vials at a regular time interval and analyzed for concentrations in the effluents.

Nitin Khandelwal, Ekta Tiwari, Nisha Singh, and Gopala Krishna Darbha. Heterogeneously Porous Multiadsorbent Clay–Biochar Surface to Support Redox-Sensitive Nanoparticles: Applications of Novel Clay–Biochar–Nanoscale Zerovalent Iron Nanotrident (C-BC-nZVI) in Continuous Water Filtration.

ACS ES&T Water, 23/02/2021; https://doi.org/10.1021/acsestwater.0c00147 

Keywords: remediation, nanocomposite, REE extraction, toxic metal sorption, dye removal, water purification, nanoadsorbent, column separation

2. LAMBDA OMNICOLL Fraction Collector enabled the sampling of defined depth intervals. It is positioned in a glovebox using Argon to prevent re-oxidation of the samples.

Henning Schroeder,Lars Duester,Anne-Lena Fabricius,Dennis Ecker,Vera Breitung,Thomas A. Ternes. Sediment water (interface) mobility of metal(loid)s and nutrients under undisturbed conditions and during resuspension. 

Journal of Hazardous Materials, 19/03/2020; https://doi.org/10.1016/j.jhazmat.2020.122543 

Keywords: Pore water depth profiles, ICP-QQQ-MS, Peeper, Mobilization, Multi-element 

3. Perifusate fractions were collected using an OMNICOLL automated fraction collection system into 96-well MASTERBLOCK plates (Greiner bio-one) and at the end of the ex- periment stored at −80 °C

Barlow, Jonathan et al. Conditioned media from contracting skeletal muscle potentiates insulin secretion and enhances mitochondrial energy metabolism of pancreatic beta-cells Metabolism.

Clinical and Experimental, Volume 91, 1 – 9; https://doi.org/10.1016/j.metabol.2018.11.004  

Keywords: Pancreatic beta-cell insulin secretion, Type 2 diabetes, Mitochondrial function, Exercise, Skeletal muscle, Organ crosstalk

4. To study the impact of mechanical disturbance and acidification on the metal(loid), OMNICOLL fraction collector is used to collect 12 profiles of 22 samples during each experiment. 

Henning Schroeder, Anne-Lena Fabricius, Dennis Ecke r, Thomas A. Ternes, Lars Duester, Impact of mechanical disturbance and acidification on the metal(loid) and C, P, S mobility at the sediment water interface examined using a fractionation meso profiling ICP-QQQ-MS approach. 

Federal Institute of Hydrology, Division G – Qualitative Hydrology, Am Mainzer Tor 1, 56068 Koblenz, Germany

Keywords: Metal release, Pore water depth, profile Colloids Size, fractionation, Sediment water interface, Metalloid release.

5. OMNICOLL fraction collector was used to collect the elute in 1.2 − 3.6 mL fractions from the contaminated sediments packed column to investigate the mobilization of Tc under fully saturated seawater flow conditions
Jane Eagling, Paul J. Worsfold, William H. Blake, and Miranda J. Keith-Roach, Mobilization of Technetium from Reduced Sediments under Seawater Inundation and Intrusion Scenarios, Environ. Sci. Technol. 2012, 46, 11798−11803
Plymouth University, UK
Keywords: Column, HPLC, Fe-reducing sediments, Tc, polyetheretherketone (PEEK) self-pack column, Eh, Sediment reoxidation

6. Effluent collected by OMNICOLL fraction collector from the glass column filled with Bt horizon of a natural soil and analyzed by ion chromatography to study the sorption of major cations (Ca, Na) on a natural sediment
Jun Lu, Catherine Beaucaire, Emmanuel Tertre, Predictive model for migration of metallic cations in natural sediments, Procedia Earth and Planetary Science 7 ( 2013 ) 529 – 532.
CEA, DANS/DPC/SECR/L3MR and Université de Poitiers-CNRS, France.
Keywords: reactive transport model; metallic cation; sediment; ion exchange model; reversible sorption

7. Eluate was collected (60 min per fraction) by means of a LAMBDA OMNICOLL fraction collector to determine the silver ion release from coated catheters
Aylvin Jorge Angelo Athanasius Dias, Edith Elisabeth M. Van Den Bosch, Astrid Franken (2010), Antimicrobial coating, US Patent no. US 2010/0113871 A1.

8. Effluent from the glass chromatography column was collected by the OMNICOLL fraction collector to study ion-exchange reactions between Na+, H+, and Ca2+ under dynamic conditions
Jun Lu, Emmanuel Tertre, Catherine Beaucaire, Assessment of a predictive model to describe the migration of major inorganic cations in a Bt soil horizon, Applied Geochemistry, Volume 41, February 2014, Pages 151-162.
CEA, DANS/DPC/SECR/L3MR and Université de Poitiers-CNRS, France.
Keywords: Ion-exchange reactions; Cationic Exchange Capacity (CEC); Ion chromatography; Bt horizon; Packed column; Wyoming montmorillonite

9. OMNICOLL fraction collector was used to collect the eluent from the contaminated sediment packed PEEK column to determine the pH and 90Sr by liquid scintillation
Eagling, Jane, The effect of sea level rise on radionuclide mobility at contaminated nuclear sites, 2012.
Plymouth University, UK
Keywords: contaminated land, porewater salinization, radionuclide, geochemistry, release kinetics, transport, oxic and reduced sediments

10. Effluent samples were collected from the fixed bed column by the multi-channel LAMBDA OMNICOLL fraction collector to study the adsorption of selenite and selenate by Mg-Al-CO3 LDH in the continuous flow system
Chubar, Natalia, and Małgorzata Szlachta., Static and dynamic adsorptive removal of selenite and selenate by alkoxide-free sol–gel-generated Mg–Al–CO3 layered double hydroxide: Effect of competing ions, Chemical Engineering Journal 279 (2015): 885-896.
Utrecht University, The Netherlands; Glasgow Caledonian University, UK and Wrocław University of Technology, Poland.
Keywords: Selenite, Selenate, Mg-Al layered double hydroxide, Batch adsorption, Dynamic adsorption, FTIR

11. Automated sample collector OMNICOLL was used to collect effluent samples (10 ml/min) from the packed bed column to evaluate the transport potential of stabilized milled ZVI particle suspensions
Velimirovic, Milica, Doris Schmid, Stephan Wagner, Vesna Micić, Frank von der Kammer, and Thilo Hofmann, Agar agar-stabilized milled zerovalent iron particles for in situ groundwater remediation, Science of The Total Environment (2015)
Helmholtz-Centre for Environmental Research - UFZ, Germany; University of Vienna, Austria
Keywords: Milled zerovalent iron, Agar agar, Particle stability, Particle transport, Particle reactivity

12. To develop a technology for extraction of Uranimum from sulphate chloride OMNICOLL fraction collector was used to collect samplings of filtrate in combination with multichannel peristaltic pump. 

Svetlana Mikhailovna TITOVA, Development of technology for sorption extraction of uranium from sulphate-chloride solutions of in-situ leaching: dissertation for the degree of candidate of technical sciences: 05.17.02.

Department of Rare Metals and Nanomaterials Institute of Physics and Technology

13. LAMBDA OMNICOLL connected to a HPLC column - by using a valve and a capillary - for collecting 2 ml fractions in 96 small scintillation vials (capacity of ~6 mL).

Gaugler, P., Gaugler, V., Kamleitner, M. & Schaaf, G. (2020). Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC. Department of Plant Nutrition, Institute of Crop Science and Resource Conservation, University of Bonn; https://doi.org/10.3791/61495 

Keywords: SAX-HPLC column, valve, scintillation vials, 2 ml fractions

14. During the firn core melting process in a clean booth (ISO 5), the remaining sample stream from the debubbler was collected with a liquid fraction collector LAMBDA OMNICOLL (~0.7 mL/min) as an archive of the meltwater.

Seokhyun Ro, S., Hur, S. D., Hong, S., Chang, Ch., Moon, J., Han, Y., Jun, S. J., Hwang, H. & Hong, S. (2020). An improved ion chromatography system coupled with a melter for highresolution ionic species reconstruction in Antarctic firn cores. Microchemical Journal, Elsevier, MICROC 105377; https://doi.org/10.1016/j.microc.2020.105377 

Keywords: on-line multi-ion chromatography system; firn core melter; fluoride ion; methanesulfonate ion; Styx Glacier; Antarctica. 

Může OMNICOLL nahradit sběrač frakcí LKB Superfrac (GE HealthCare), který již není dále k dispozici?

Ano, sběrač frakcí a vzorkovač OMNICOLL může nahradit Superfrac (také sběrač frakcí Frac-950, Frac-920).

 Prodáváte také různé nástavce pro sběrač, které by umožňovaly sběr do více nádob najednou?

Nabízíme přizpůsobení i pro sběr frakcí do více nádob, stojanů, … najednou podle přání zákazníka. Jedná se o vícekanálovou konfiguraci.

 Máte zkušenosti s ovládáním vícekanálové peristaltické pumpy Ismatec IPC s OMNICOLLem?

Společnost LAMBDA nabízí kabel dálkového ovládání pro vícekanálové čerpadlo Ismatec pro analogovou komunikaci mezi sběračem OMNICOLL a pumpou.

 Je možné použít jakoukoliv peristaltickou pumpu se sběračem frakcí LAMBDA OMNICOLL?

Ano, je možné použít jakoukoliv peristaltickou pumpu se sběračem frakcí LAMBDA OMNICOLL.

Sběrač frakcí OMNICOLL poskytuje signál (~ 9 V), který je použit komunikačním modulem (číslo výrobku 6911) k vypnutí pumpy při přejezdu z jedné frakce ke druhé a na konci běhu. Proto nedochází k rozlití mezi frakcemi.

Navíc při použití sběrače frakcí OMNICOLL s PERISTALTICKÝM ČERPADLEM LAMBDA může sběrač frakcí odebírat frakce objemově pomocí počítání impulsů pumpy (režim count). Každá frakce bude mít stejné množství impulzů a bude mít tedy i stejný objem.

 Jaký je nejvyšší průtok, pro který je sběrač frakcí OMNICOLL ještě vhodný?

Sběrač a vzorkovač frakcí OMNICOLL je flexibilní systém, který lze přizpůsobit požadované aplikaci.

Rychlost průtoku závisí na rozdílu tlaku a na průměru použité hadičky. Je možné vybrat hadičku a průměr podle požadavků.