Fermentatorul/Bioreactorul de laborator LAMDA MINIFOR introduce concepte noi despre culturile celulare si fermentatia in laborator:

Fermentatorul/Bioreactorul de laborator MINIFOR

  • Conceptul de sterilizare usoara.
  • Control automat al parametrilor: pH, O2, DO, presiune.
  • Noua linie de sticlarie cu garnituri si gat filetat maresc flexibilitatea utilizarii.
  • Volumul culturilor intre 35 ml si 6 L intr-un singur instrument.
  • Razele IR determina incalzirea precisa, economica a culturilor, nemaifiind necesara baia de apa.
  • Compact
  • Noul agitator biomimiking tip "peste-coada" pentru o agitare eficienta a culturilor
  • Controlul debitului de gaz prin masurarea exacta a debitului de masa.
  • Control automat anti-spuma.
  • Foarte rapid, usor de configurat si demontat
  • Sterilizabil in autoclave obisnuite
  • Costructie din materiale moderne
  • Posibilitate conectare PC
  • Posibilitate procese fermentatie in paralel
  • Fermentatorul / bioreactorul LAMBDA MINIFOR este controlat de două microprocesoare.
Alimentare 100-240 V AC/50-60Hz, 510W, conform CE
Dimensiuni 22 × 40 × 38 cm (W x D x H)
Display LCD 4 × 40 de caractere, luminos
Vas fermentator Sticlă pyrex cu gât lateral 5-8 l 0,3, 0,4, 1, 3 şi 7
Volum De la 35 ml la 6 L
Controlul Temperaturii Radiatie IR pana la peste 70°C in pasi de 0,1°C
Precizie ±0,2°C
Senzor Pt100 incorporat in senzorul de pH
Controlul pH Electrod de pH Metttler Toledo, sterilizabil, corectia temperaturii automata, conector Variopin, calibrare semiautomata in doua puncte.
Rezolutie 0,01
Precizie ±0,2°C
Pompa Pot fi folosite independent pana la 4 pompe peristaltice (PRECIFLOW, MULTIFLOW, HIFLOW sau MAXIFLOW)
Controlul O2 Senzor de oxigen sterilizabil, corectie automata a temperaturii.
Domeniu 0-25 mg O2/l, in pasi de 0,1 mg/l
Debit aer 0-5 l / min. în pasi de 0,01 l / min.
linearitate ± 3%
reproductibilitate ± 5%
Presiune aer furnizat 0,05 – 0,2 MPa
Agitare 50 W Vibromixer 0-20 Hz (0-1200 rpm), în paşi de 0,1 Hz, cu 1 sau mai multe discuri de agitare
Parametri selectabili suplimentar poate fi controlat unul din parametrii: PCO2, densitatea optică, conductivitate, etc)
Prelevare/probe suplimentare pentru prelevarea de probe şi adăugarea de soluţii de corecţie pot fi folosite pana la 4 ace din oţel inoxidabil cu accesoriile LAMBDA PEEK
Temperatura de lucru 0-40 ° C
Umiditate relativă de lucru 00 - 90% RH (fără condensare)
Greutate 7,5 kg
control PC folosind FNet sau software-ul SIAM (opţional)
Garantie 2 ani
Power: Universal power supply for mains 100-245 V AC/50-60Hz, 560W, CE conform
Dimensions: 22 x 40 x 38 cm (W x D x H)
Display: LCD 4 x 40 digits with backlight illumination
Fermentor vessel: Pyrex glass with 6 to 8 side necks; 0.3, 0.4, 1, 3, 7 liter vessels
Temperature control: High efficiency 150 W infrared (IR) radiation heat source with gilded parabolic reflector
Regulation: from 5°C over RT to 70°C
Measurement: from 0 to 99.9°C in 0.1°C steps
Precision: +/- 0.2°C (0 to 60°C)
Sensor: Pt 100 incorporated in the glass electrode of the pH probe
pH control: sterilisable pH electrode pH 0-14 with automatic temperature correction, two-point semiautomatic calibration and Variopin connector
Resolution: 0.01 pH unit
Precision: +/- 0.02 pH unit
pO2 control: sterilisable Clark type oxygen sensor with fast response, automatic temperature correction, two-point semiautomatic calibration, dissolved oxygen (DO) control through regulation of the airflow rate
Range: 0 to 25 mg oxygen/ l, in 0.1 mg/l steps
Air flow: 0 to 5 l/min in 0.01 l/min steps, measured by precise mass flow meter, linearity +/- 3%, reproducibility +/- 0.5%
Control: proportional valve controlled by microprocessor
For supplied air pressure: 0.05 – 0.2 MPa (0.5 - 2 atm)
Agitation: 50 W Vibromixer 0 to 20 Hz (0 to 1200 rpm) in 0.1 Hz steps (6 rpm) with 1 or more stirring discs; Sterility similar to magnetic coupling
Selectable parameter 'X': an additional parameter can be controlled by the instrument (foaming control, weight (for continuous cultures), pCO2, redox potential, conductivity, optical density, etc.); with standard 0-10V or 0-20mA output
Ports / side necks: One large quadruple sampling or additions port with four needles with LAMBDA PEEK double-seal connections, used for sampling, inoculation, antifoam, feeds, harvest, addition of correction solutions etc., additional double ports are available.
Pumps: up to 4 independent pumps (PRECIFLOW, MULTIFLOW, HIFLOW or MAXIFLOW) with speed variation from 0 to 100 % can be used with MINIFOR lab fermenter-bioreactor
Gas flow control: In addition to pumps, several electronic flow controllers with flow rate ranges of 0-5 l/min (MASSFLOW 5000) or 0-500 ml/min (MASSFLOW 500) can be used for the controlled addition of gases (e.g. N2, O2, air, CO2) in cell cultures; freely configurable gas station module
Working temperature: 0 – 40 °C
Working humidity: 0 - 90 % RH, not condensing
Weight: 7.5 kg
PC control: complete PC control and data processing using the fermentation software FNet (for up to 6 MINIFOR fermenters) or SIAM (for an even higher number of instruments)


Submerged batch (consortia) and fed-batch (L. coryniformis) fermentations in LAMBDA MINIFOR stirred-tank bioreactor and SIAM software: 72 h, final working volumes = 200 ml, T = 37 ± 0.5 °C, stirring 0.5 Hz, sparing CO2 at 0.04 L/min, pH 6.5 ± 0.5 using 1 M NaOH and 1 M H2SO4 for automatic pH adjustment; samples were taken in regular intervals.

Buljubašić, E., Bambace, M. F., Christensen, M. H. L., Ng, K. S., Huertas‐Díaz, L., Sundekilde, U., Marietou, A. & Schwab, C. (2024). Novel Lactobacillaceae strains and consortia to produce propionate‐containing fermentates as biopreservatives. Microbial Biotechnology, 17(2), e14392.

The in vitro multispecies dynamic biofilm model was used which has been validated on biofilms growing on implant surfaces: LAMBDA MINIFOR bioreactor maintains the protein-enriched BHI medium under stable conditions: 37 °C, pH 7.2 and an anaerobic atmosphere by directly pumping an anaerobic gas mixture (10% H2, 10% CO2 and equilibrium N2) keeping the pressure constant during the whole incubation process

Bravo, E., Arce, M., Ribeiro-Vidal, H., Herrera, D., & Sanz, M. (2024). The Impact of Candida albicans in the Development, Kinetics, Structure, and Cell Viability of Biofilms on Implant Surfaces. An In Vitro Study with a Validated Multispecies Biofilm Model. 

Simulated human colon model LAMBDA MINIFOR with basal nutrient medium (37 °C, pH 6.8) and faecal slurries

Pusuntisumpun, N., Tunsagool, P., Nitisinprasert, S., & Nakphaichit, M. (2024). Impacts of combining Limosilactobacillus reuteri KUB‐AC5 and Limosilactobacillus fermentum KUB‐D18 on overweight gut microbiota using a simulated human colon model. International Journal of Food Science & Technology.

400 ml isolated tomato cells in a LAMBDA MINIFOR bioreactor (20 °C, pH 5.8) got sparged (10 L/h) to achieve varied O2 concentrations (21 kPa, 5 kPa, and 0 kPa) for the study of postharvest losses of fruits and vegetable during controlled atmosphere storage as a result of low O2 stress.

Mahomud, M. S., Islam, M., & Roy, J. (2024). Effect of low oxygen stress on the metabolic responses of tomato fruit cells. Heliyon, e24566.

Continuous itaconic acid (IA) fermentations with A. terreus spp.: LAMBDA MINIFOR 3L autoclavable bench-top laboratory fermenter (working volume 1.8 litres; dilution rate 0.007 h-1; 33 °C; controlled low pH; one week 1.5 vvm air, then 0.2 vvm O2) with septum for sterile sampling and LAMBDA FiSH-TAIL agitator (3.0 s-1) providing gentle and efficient mixing for optimal oxygenation and gas exchange in absence of air flooding which are advantageous properties when working with filamentous microorganisms to achieve clump growth as the most efficient morphology for IA production.

Hülber-Beyer, É. A., Nemestóthy, N., & Bélafi-Bakó, K. (2023). Case Study of Continuous Itaconic Acid Fermentation by Aspergillus Terreus in a Bench-Scale Bioreactor. Hungarian Journal of Industry and Chemistry, 51(2), 57-63.

Pictures of morphology during fermentation: Fig. 1 on (2024 Feb. 08) 
Simultaneous saccharification (enzymatic hydrolysis with cellulase and β-glucosidase) and fermentation (Lacticaseibacillus rhamnosus) of pretreated delignified lignocellulosic plum biomass to lactic acid in a LAMBDA MINIFOR Advanced Kit bioreactor (1.7 L; 37 °C /44 °C; pH 5.5 / 6.5; 72 h).

Senila, L., Cadar, O., Kovacs, E., Gal, E., Dan, M., Stupar, Z., Simedru, D., Senila, M. & Roman, C. (2023). L-Poly(lactic acid) Production by Microwave Irradiation of Lactic Acid Obtained from Lignocellulosic Wastes. Int. J. Mol. Sci. 2023, 24, 9817. 

Bacillus megaterium fermentation performed in bioreactor LAMBDA MINIFOR (1.7 L)

Senila, L., Gál, E., Kovacs, E., Cadar, O., Dan, M., Senila, M. & Roman, C. (2023). Poly(3-hydroxybutyrate) Production from Lignocellulosic Wastes Using Bacillus megaterium ATCC 14581. Polymers. 2023, 15, 4488. 

In vitro multispecies dynamic biofilm model using LAMBDA MINIFOR for inolculation and growth of bacteria (Streptococcus oralis, Veillonella parvula, Actinomyces naeslundii, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis) in anaerobic atmosphere [10% H2, 10% CO2, balance N2]) at 37°C and pH 7.2, followed by transfer to a Robbins device with dental hybrid titanium implants (HS) with different topographies for biofilm formation.

Bravo, E., Serrano, B., Ribeiro-Vidal, H., Virto, L., Sánchez, I.S., Herrera, D. & Sanz, M. (2023). Biofilm formation on dental implants with a hybrid surface microtopography: An in vitro study in a validated multispecies dynamic biofilm model. John Wiley & Sons, Ltd., 0905-7161. Clinical Oral Implants Research, Volume 34, Issue 5, May 2023 Pages i-iii, 405-541. 
Simulation of in-vitro gut model with LAMBDA MINIFOR 0.3L: Batch fermentations under anaerobic conditions (37 °C, pH 6.8–6.9, 24 h) of human fecal slurry (1% (v/v)) to evaluate the effect of Triphala extracts (1 mg/mL) co-fermentation on microbiota and metabolic changes.

Kwandee, P., Somnuk, S., Wanikorn, B., Nakphaichit, M. & Tunsagool, P. (2023). Efficacy of Triphala extracts on the changes of obese fecal microbiome and metabolome in the human gut model. Journal of Traditional and Complementary Medicine, Volume 13, Issue 2, 2023, Pages 207-217, ISSN 2225-4110.

Gastrointestinal Simulation Model SHIME with LAMBDA MINIFOR 0.3L reactors:
Each Simulator of Human Intestinal Microbial Ecosystem consists of five reactors, mimicking different sections of the human Gastrointestinal tract.

The video shows Assoc. Prof. Massalin Nakphaichit in front of the SHIME reactors.

Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University

In a bench-scale LAMBDA MINIFOR 7L bioreactor with programmable probes, RODMs (a set of organisms with novel metabolism, efficiently degrading highly-concentrated aromatics) were developed for a high-density microbial ecosystem.

Ahmad, M., Yousaf, M., Han, J.-C., Huang, Y., Zhou, Y. & Tang, Z. (2023). Development of Biocatalytic Microbial Ecosystem (FPUS@RODMs@In-PAOREs) for Rapid and Sustainable Degradation of Various Refractory Organics. Journal of Hazardous Materials, 2023, 131514, ISSN 0304-3894,

Hybridoma cell (inoculation 4.0×10E5 cells/mL (90% viability)): Fed-batch in a LAMBDA MINIFOR stirred-tank bioreactor.

Llamo, A., Hernández, D., García, C., González, M., Ferro, W., Garay, H., Diago, D., Fajardo, A., Espinosa, L., Padilla, S., Gómez, L., Chinea, G. & and Valdés, R. (2023). Gamma-Immunoglobulin Response Characterization, in COVID-19 Convalescent Patients, Against the Spike Protein S2 Subunit with Eight Linear Peptides for Monoclonal Antibody Generation. BioProcess J, 2023; 22.

Optimization of lipase-catalyzed hydrolysis of vegetable oils: Control and monitoring by a LAMBDA MINIFOR bioreactor (pH, temperature, reaction time, enzyme load and oil/aqueous ratio of the reaction mixture).

Faillace, E., Brunini-Bronzini de Caraffa, V., Mariani, M., Berti, L., Maury, J. & Vincenti, S. (2023). Optimizing the First Step of the Biocatalytic Process for Green Leaf Volatiles Production: Lipase-Catalyzed Hydrolysis of Three Vegetable Oils. International Journal of Molecular Sciences. 2023; 24(15):12274.

Evaluation of viability and maximum specific growth rate of Bacillus licheniformis in a bench-top LAMBDA MINIFOR 7L laboratory bioreactor (2 litres working volume, 37 °C, pH 6.5, 200 rpm, controlled oxygen, submerged batch fermentation (SMF), addition of sterilized Antifoam 204 agent (Sigma-Aldrich))

Dumitru, M. & Ciurescu, G. (2023). Optimization of the fermentation conditions and survival of Bacillus licheniformis as freeze-dried powder for animal probiotic applications. Scientific Papers. Series D. Animal Science. Vol. LXVI, No. 2, 2023; ISSN 2285-5750; ISSN CD-ROM 2285-5769; ISSN Online 2393-2260; ISSN-L 2285-5750. (2024 Jan. 02)

LAMBDA MINIFOR 0.4L bioreactor for medium conditioning (37 °C, pH 7.2, constant anaerobic conditions (10% H2, 10% CO2, N2)) with weighing module and medium pumps (30 ml/h) in a dynamic in vitro biofilm model for mimicking the oral cavity environment.

Alonso-Español, A., Bravo, E., Ribeiro-Vidal, H., Virto, L., Herrera, D., Alonso, B. & Sanz, M. (2023). The Antimicrobial Activity of Curcumin and Xanthohumol on Bacterial Biofilms Developed over Dental Implant Surfaces. Int. J. Mol. Sci. 2023, 24, 2335.

Itaconic acid (IA) production by continuous aerobic fungal fermentation (1.8 L, 37 °C, 0.2 vvm pure oxygen, pH naturally reduced to <2.5) in a LAMBDA MINIFOR bioreactor with native itaconic acid overporducing Aspergillus terreus NRRL 1966 using high glucose concentration (maintained at ~150 g/L) as carbon source.
Rózsenberszki, T., Komáromy, P., Hülber-Beyer, E., Pesti, A., Koók, L., Bakonyi, P., Bélafi-Bakó, K. & Nemestóthy, N. (2023). Bipolar membrane electrodialysis integration into the biotechnological production of itaconic acid: A proof-of-concept study. Chemical Engineering Research and Design, Volume 190, 2023, Pages 187-197, ISSN 0263-8762.

Milk was pasteurized at 70 °C for 30 min in LAMBDA MINIFOR fermenters
LAMBDA MINIFOR fermenters: Temperature effect tests on growth of yeast Kazachstania unispora (initial ~10E6 CFU/ml) in milk (800 ml, ~6 % lactose): from 5 °C to 40 °C (5, 10, 15, 20, 25, 27, 30, 32, 35, 37, and 40 °C) at pH 5.6 (automatic adjustment with 2 M NaOH) and 240 rpm, until stationary phase was reached (inline near-infrared turbidity sensor Optek FC20- ASD10-N)
LAMBDA MINIFOR fermenters for coculture experiments at 25 °C of Lacticaseibacillus casei and Kazachstania unispora in modfied MRS media as well as in mare milk (initial: ~10E6 CFU/ml, pH = 6.8)
LAMBDA MINIFOR fermenters for coculture experiments at 30 °C of Lactobacillus kefiri and Kazachstania unispora in modfied MRS media as well as in mare milk (initial: ~10E6 CFU/ml, pH = 6.8)

Kondybayev, A., Achir, N., Mestres, C., Collombel, I., Strub, C., Grabulos, J., Akhmetsadykov, N., Aubakirova, A., Kamidinkyzy, U., Ghanmi, W. & Konuspayeva, G. (2023). Growth Kinetics of Kazachstania unispora and Its Interaction with Lactic Acid Bacteria during Qymyz Production. Fermentation 2023, 9, 101. 

Efficient ethanol production from whey permeate (WP) and concentrated permeate (CWP) with engineered E. coli in pH-controlled bioreactor LAMBDA MINIFOR

Pasotti, L., De Marchi, D., Casanova, M., Frusteri Chiacchiera, A., Cusella De Angelis, M. G., Calvio, C., & Magni, P. (2023). Design of a stable ethanologenic bacterial strain without heterologous plasmids and antibiotic resistance genes for efficient ethanol production from concentrated dairy waste. Biotechnology for Biofuels and Bioproducts, 16(1), 1-13. 

Production of biosurfactant (BS, 32 g/L ) by Lactobacillus acidophilus in a laboratory bioreactor LAMBDA MINIFOR 7L (3.5 litres working volume, 30 °C, pH = 6.5, 120 h batch culture), purfication of supernatant and BS studies.
Abdullah, A.R. & Ismail, H.H.(2022). Cytotoxic effect of biosurfactant produced by Lactobacillus acidophilus and study its synergistic effect with certain antibiotics against S. aureus and E. coli. Eurasian Medical Research Periodical, 12, 33–40. (2022 Sept. 22)

LAMBDA MINIFOR bioreactor and fermentor for schooling & university 
Technologische Fachoberschule Bruneck, Fachrichtung Chemie, Werkstoffe und Biotechnologie (2021). Bioreaktor. (2022 September 13)
Lactic acid bacteria growth experiments: Effect of temperature on Lacticaseibacillus casei and Lactobacillus kefiri.

Kondybayev, A.; Konuspayeva, G.; Strub, C.; Loiseau, G.; Mestres, C.; Grabulos, J.; Manzano, M.; Akhmetsadykova, S. & Achir, N. (2022). Growth and Metabolism of Lacticaseibacillus casei and Lactobacillus kefiri Isolated from Qymyz, a Traditional Fermented Central Asian Beverage. Fermentation 2022, 8, 367.

During 65 days, two continuous (HRT= 5 days) stirrer tank fermenters LAMBDA MINIFOR were operated under anaerobic conditions (N2 into headspace & sparging), each with 1 liter working volume (modification of lactate / acetate concentrations) inoculated with caproate-producing sludge (Caproiciproducens genus (Ruminococcaceae family)), temperature control (30 °C, build-in IR heater, Mettler InPro 3253 probe) and pH control (pH 5.5, NaOH 2M, HCl 0.5M) ) with four peristaltic pumps (feed, effluent, base & acid) and daily liquid sampling for carboxylates and alcohols analysis.
Brodowski, F., Lezyk, M., Gutowska, N., Kabasakal, T. & Oleskowicz-Popiel, P. (2022). Influence of lactate to acetate ratio on biological production of medium chain carboxylates via open culture fermentation. Science of The Total Environment, Volume 851, Part 1, 2022, 158171, ISSN 0048-9697.

The continuous culture was performed in a Lambda Photobioreactor (PBR). White light from the LAMBDA LUMO module was calibrated to umolm−2 m−1. For evaporation control and continuous culture mode, the total weight of the reactor setup was kept constant using the built-in LAMBDA reactor mass control module and automatic addition of fresh culture medium through the feed pump. Continuous culture was performed by setting the waste pump to a fixed speed.

Behle, A., Dietsch, M., Goldschmidt, L., Murugathas, W., Berwanger, L.C., Burmester, J., Yao, L., Brandt, D., Busche, T., Kalinowski, J., Hudson, E.P., Ebenhöh, O., Axmann, I.M. & Machné, R. (2022). Manipulation of topoisomerase expression inhibits cell division but not growth and reveals a distinctive promoter structure in Synechocystis. Nucleic Acids Research, Volume 50, Issue 22, 9 December 2022, Pages 12790–12808. 

Biocatalytic resolution of lupanine racemate in industrial wastewater by Pseudomonas putida LPK411 using a lab‐scale bioreactor LAMBDA MINIFOR 0.4L under batch operation.

Parmaki, S., Esteves, T., Gonçalves, J.M.J. Catenacci, A., Malpei, F., Ferreira, F.C., Afonso C.A.M & Koutinas, M. (2022). Selective microbial resolution of lupanine racemate: Bioprocess development and the impact of carbon catabolite repression on industrial wastewater valorisation. Biomass Conv. Bioref. (2022). 

For releasing bioactive peptides, diluted Kiwicha protein isolate (KPI) from Amaranthus caudatus L. seed flour was subjected to enzymatic hydrolysis with Bioprotease LA-660 using a LAMBDA MINIFOR bioreactor for continuous stirring, pH- and temperature control (pH 8; 50 °C).

Martinez-Lopez, A., Rivero-Pino, F., Villanueva, A., Toscano, R., Grao-Cruces, E., Marquez-Paradas, E., Martin, M.E., Montserrat-de la Paz, S. & Millan-Linaresa, M.C. (2022). Kiwicha (Amaranthus caudatus L.) protein hydrolysates reduce intestinal inflammation by modulating the NLRP3 inflammasome pathway. Food & Function 2022 Oct 21. 

LAMBDA MINIFOR fermenters with weighing modules to control the harvest pumps for the continuous anaerobic biotechnological process were used to verify how the external acetate affects the product spectrum, gas production, stability and efficiency of carboxylates production.
Brodowski, F., Lezyk, M., Gutowska & Oleskowicz-Popiel, P. (2022). Effect of external acetate on lactate-based carboxylate platform: Shifted lactate overloading limit and hydrogen co-production. Science of The Total Environment, Volume 802, 2022, 149885, ISSN 0048-9697.

Escherichia coli (E. coli; E44Δ) mutant strain for production of large quantity of outer membrane vesicles (OMVs) in a LAMBDA MINIFOR 7L fermenter. 

Allahghadry, T., Bojesen, A.M., Whitehead, B.J. and Antenucci, F. (2022). Clarification of large-volume bacterial cultures using a centrifuge-free protocol. J Appl Microbiol. Accepted Author Manuscript. 



Experiments on liquid phase (hemicelluloses hydrolysate) for xylitol production: The fermentation of 250 ml of detoxified hydrolysate was conducted in a 1L fermenter (LAMBDA MINIFOR bench-top-laboratory-fermenter) and pH adjustment (pH 5.0) at aerobic conditions at 30 °C for 60 hr. 

Shalsh, D., Nagimm, D., Alrheem, M.A. & Alrheem, S.A. (2021). Batch fermentation and Simultaneous Saccharification and Fermentation (SSF) processes by Meyerozyma Guilliermondii Strain F22 and Saccharomyces cerecvisae for xylitol and bioethanol co-production. Al-Qadisiyah Journal of Pure Science, 26(4), 80–94. 

The growth, glucose consumption and ethanol production of Saccharomyces cerevisiae LM strain in synthetic broth were modeled for the most important intrinsic...One liter Lambda Minifor fermenters equipped with a cold water condenser on air exit pipes (LAMBDA Instruments GmbH, Baar- Switzerland), were used.

Kouamé, C., Loiseau, G., Grabulos, J., Boulanger, R. & Mestres, C. (2021). Development of a model for the alcoholic fermentation of cocoa beans by a Saccharomyces cerevisiae strain. International Journal of Food Microbiology, Volume 337, 2021, 108917, ISSN 0168-1605. 

Continuous culture of cyanobacteria Synechocystis sp. PCC 6803 in a LAMBDA MINIFOR 1L PBR photo-bioreactor. 

Behle, A., Dietsch, M., Goldschmidt, L., Murugathas, W., Brandt, D., Busche, T., Kalinowski, J., Ebenhöh, O., Axmann, I. M. & Machné, R. (2021) Uncoupling of the Diurnal Growth Program by Artificial Genome Relaxation in Synechocystis sp. PCC 6803. bioRxiv 2021.07.26.453758. 

The hydrolysis of kiwicha protein isolate (KPI) is performed under continuous stirring, using a LAMBDA MINIFOR fermenter-bioreactor, at controlled conditions of pH and temperature: Bioprotease LA-660 is added at a ratio enzyme/substrate = 0.3 AU/g protein (pH 8) for 5, 10, 15, 30, and 60 min.

Paz, S. M. D. L., Martinez-Lopez, A., Villanueva-Lazo, A., Pedroche, J., Millan, F., & Millan-Linares, M. C. (2021). Identification and characterization of novel antioxidant protein hydrolysates from kiwicha (Amaranthus caudatus L.). Antioxidants, 10(5), 645.

The biological transformation of white sorghum biomass was performed under operating conditions similar to the MixAlco process. MixAlco batch fermentation process were performed in the LAMBDA MINIFOR bioreactor.

Shalsh, F.J., Alrazzaq, N.A., Nagimm D.K., Alrheem, M.A., Alrheem S.A. & Abd-alalah, K. (2021). Bioconversion of white sorghum biomass using MixAlco fermentation process. DYSONA – Applied Science. 2021(2), 21-27. ISSN 2708-6283.



Different yeast strains were cultivated in the LAMBDA MINIFOR 0.4L Fermentor to study the metabolic cylce and pathway. 

J. Feltham, S. Xi, S. Murray, M. Wouters, J. Urdiain-Arraiza, C. George, A. Townley, E. Roberts, R. Fisher, S. Liberatori, S. Mohammed, B. Kessler & J. Mellor. (2020). Transcriptional changes are regulated by metabolic pathway dynamics but decoupled from protein levels. bioRxiv 833921.

LAMBDA MINIFOR Bioreactor is used as a Rumen membrane bioreactor to produce volatile fatty acids (VFA) from crop residues (lignocellulosic biomass) by mimicking the digestive system of ruminant animals.

Nguyen, A.Q., Nguyen, L.N., Abu Hasan Johir, M., Ngo, H-H., Chaves, A.V. & Nghiem, L.D. (2020) Derivation of volatile fatty acid from crop residues digestion using a rumen membrane bioreactor: a feasibility study. Bioresource Technology 2020.

Enzymatic hydrolysis experiments were carried out in the lab-scale LAMBDA MINIFOR stirred-batch bioreactor. The pretreated vine-shoot waste was delignified with sodium chlorite for lignin removal and then enzymatically hydrolyzed using new types of enzymes (cellulase from Trichoderma reesei and b-glucosidase).

Eniko Kovacs, Daniela Alexandra Scurtu, Lacrimioara Senila, Oana Cadar, Diana Elena Dumitras & Cecilia Roman (2020). Green Protocols for the Isolation of Carbohydrates from Vineyard Vine-Shoot Waste. Analytical Letters.

LAMBDA MINIFOR bioreactor used to produce Itaconic Acid biotechnologically by Aspergillus terreus fungal strain from glucose

Nemestóthy, N., Komáromy, P., Bakonyi, P. et al. (2020). Carbohydrate to Itaconic Acid Conversion by Aspergillus terreus and the Evaluation of Process Monitoring Based on the Measurement of CO2 Waste and Biomass. Valorization 2020.



LAMBDA MINIFOR bioreactor used in turbidostat experiments with recombinant cells in continuous culture operation mode

Pasotti, L., Bellato, M., Politi, N., Casanova, M., ucca, S., Gabriella, M., De Angelis, C. & Magni, P. (2019). A synthetic close-loop controller circuit for the regulation of an extracellular molecule by engineered bacteria. IEEE Trans Biomed Circuits Syst. 2019 Feb; 13(1):248-258.

pH optimization for the aerobical production of itaconic acid catalyzed by Aspergillus terreus in a LAMBDA MINIFOR bioreactor: Batch, working volume 1.8 L, medium with 120 g/L glucose substrate, 37 °C, pH 3 - pH 2.5 - pH 4 - pH 3 - pH 2.5, stirrer 2 Hz, aeration 1.5 L (STP)/min.

Komáromy, K., Bakonyi, P., Kucska, A., Tóth, G., Gubicza, L., Bélafi-Bakó, K. & Nemestóthy, N. (2019). Optimized pH and Its Control Strategy Lead to Enhanced Itaconic Acid Fermentation by Aspergillus terreus on Glucose Substrate. Fermentation 2019, 5(2), 31

Lab-Scale Production of Rhamnolipid by Pseudomonas Aeruginosa A3 using LAMBDA MINIFOR Benchtop Bioreactor.

Faqri, A. F., Hayder, N.H. & Hashim, A.J. (2019). Lab-scale production of Rhamnolipid by Pseudomonas Aeruginosa A3 and study its synergistic effect with certain antibiotics against some pathogenic bacteria. Iraqi Journal of Agricultural Sciences –2019:50(5):1290-1301. 



A large-scale pro-siRNA production method was developed in a LAMBDA MINIFOR bioreactor for high yield production of pro-siRNA 

Kaur, G., Cheung, H. C., Xu, W., Wong, J. V., Chan, F. F., Li, Y., McReynolds, L. & Huang, L. (2018). Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli. Biotechnology and Bioengineering, 115(9), 2280-2291.

LAMBDA MINIFOR lab scale bioreactor used for production of bioethanol from lignocellulosic biodegradable municipal solid waste (BMSW) under optimized conditions

Hayder, N. H., Flayeh, H. M., & Ahmed, A. W. (2018). Optimization of bioethanol production from biodegradable municipal solid waste using response surface methodology (RSM). Journal of Engineering and Sustainable Development, 22(1), 47-64. (2024 Feb. 05)



Comparison of the experimental and theoretical production of biogas. The LAMBDA MINIFOR bioreactor filled with 2 litres of inoculum was incubated anaerobically at 35 °C for 1 month. 

El Asri, O., & Afilal, M. E. (2018). Comparison of the experimental and theoretical production of biogas by monosaccharides, disaccharides, and amino acids. International Journal of Environmental Science and Technology, 15(9), 1957-1966.

Study of the metabolism of isolated lamb’s lettuce cells (Valerianella locusta (L). Laterr.) upon sugar starvation under O2 stress conditions using 13C [U-13C6] labelled glucose in a LAMBDA MINIFOR bench-top laboratory bioreactor (dark, 250 ml, pH 5.8, 18 °C, airation 10 L/h)

Victor, B. M. M., Ampofo-Asiama, J., Hertog, M., Geeraerd, A. H., & Nicolai, B. M. (2017). Metabolic profiling reveals a coordinated response of isolated lamb's (Valerianella locusta, L.) lettuce cells to sugar starvation and low oxygen stress. Postharvest Biology and Technology, 126, 23-33.

LAMBDA MINIFOR fermenters used as continuous anaerobic flow stirred digesters (CSTR) for anaerobic digestion of organic solid waste

Nakasima-López, M., Taboada-González, P., Aguilar-Virgen, Q., & Velázquez-Limón, N. (2017). Adaptación de inóculos durante el arranque de la digestión anaerobia con residuos sólidos orgánicos. Información tecnológica, 28(1), 199-208.

The effect of different temperatures on sugar starvation in cells isolated from fresh leafy vegetables was studied in LAMBDA MINIFOR bioreactor

Mbong, V. B. M., Ampofo-Asiama, J., Hertog, M. L., Geeraerd, A. H., & Nicolai, B. M. (2017). The effect of temperature on the metabolic response of lamb’s lettuce (Valerianella locusta,(L), Laterr.) cells to sugar starvation. Postharvest Biology and Technology, 125, 1-12.

LAMBDA MINIFOR bioreactor for the production of CB.Hep-1 mAb using mouse hybridoma cell culture in protein-free media

Valdés, R., Aragón, H., González, M., Hernández, D., Geada, D., Goitizolo, D., Ferro, W., Pérez, A., García, J., Masforrol, Y, Aguilar, P., Márquez, G., LaO, M., González, T., Calvo, Y., Hernández, A.,  Menéndez, G. & Tamayo, A. (2017). Mouse Hybridoma Cell Culture in a Protein-Free Medium Using a Bio-Mimicking Fish-Tail Disc Stirred Bioreactor. BioProcessing Journal, 16(1).


Robust cellulosic ethanol production from sugarcane bagasse with Saccharomyces cerevisiae ATCC 20602 in LAMBDA MINIFOR laboratory bioreactor under aerobic and anaerobic conditions with controlled redox potential measurement

Jabasingh, S. A., Lalith, D., Prabhu, M. A., Yimam, A., & Zewdu, T. (2016). Catalytic conversion of sugarcane bagasse to cellulosic ethanol: TiO2 coupled nanocellulose as an effective hydrolysis enhancer. Carbohydrate polymers, 136, 700-709.


Bioreactor system LAMBDA MINIFOR as an artificial mouth system for the growth of biofilms


S. pyogenes Cas9 protein was expressed in E. coli using a computer-controlled LAMBDA MINIFOR 3L bioreactor in batch mode followed by exponential feeding

Ménoret, S., De Cian, A., Tesson, L., Remy, S., Usal, C., Boulé, J. B., Boix, C., Fontanière, S., Crénéguy, A., Nguyen, T.H., Brusselle, L., Thinard, R., Gauguier, D., Concordet, J.-P., Cherifi, Y., Fraichard, A., Giovannangeli, C. & Anegon, I. (2015). Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins. Scientific reports, 5(1), 14410.

Fermentation of engineered microorganism in laboratory scale bioreactor LAMBDA MINIFOR for efficient conversion of lactose-to-ethanol

Pasotti, L., Zucca, S., Casanova, M., Massaiu, I., Mazzini, G., Micoli, G., Calvio, C., Cusella de Angelis, M.G. & Magni, P. (2015, August). Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology. In 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) (pp. 953-956). IEEE.

Six-species flow cell biofilm model was developed by culturing bacteria in LAMBDA MINIFOR bioreactor to evaluate the biofilm development under flow and shear conditions

Salli, K. M., & Ouwehand, A. C. (2015). The use of in vitro model systems to study dental biofilms associated with caries: a short review. Journal of oral microbiology, 7(1), 26149. 

Quantification of ribosomal proteins (RPs) from Yeast cells cultured in LAMBDA MINIFOR bioreactor and mouse embryonic stem cells (ESC) to study the core RPs stoichiometry

Slavov, N., Semrau, S., Airoldi, E., Budnik, B., & van Oudenaarden, A. (2015). Differential stoichiometry among core ribosomal proteins. Cell reports, 13(5), 865-873. 

Harvard University, USA; Broad Institute of MIT and Harvard, USA and Hubrecht Institute, Netherlands.


Cultivation of microalgae (Chlorella vulgaris Beyerinck) in laboratory bioreactor LAMBDA MINIFOR

Heitur, H. (2014). Mikrovetika Chlorella vulgaris Beyerincki kasvatamine CO2 sidumise eesmärgil (Master's thesis). (2024 Feb. 05)

Eesti Maaülikool (Estonian University of Life Sciences), Estonia.

Growing yeast cultures (DBY12007) in the LAMBDA MINIFOR fermenter at steady state to study the aerobic glycolysis and energy flux

Slavov, N., Budnik, B. A., Schwab, D., Airoldi, E. M., & van Oudenaarden, A. (2014). Constant growth rate can be supported by decreasing energy flux and increasing aerobic glycolysis. Cell reports, 7(3), 705-714. 

Massachusetts Institute of Technology, USA; Harvard University, USA; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, Netherlands and Princeton University, USA.

Selective and non-selective batch fermentation of date extract using Saccharomyces cerevisiae (commercial strain used in bakeries (wild strain), glucose selective strains ATCC 36858 and ATCC 36859) studied in LAMBDA MINIFOR fermentor

Putra, M. D., Abasaeed, A. E., Zeinelabdeen, M. A., Gaily, M. H., & Sulieman, A. K. (2014, April). Selective fermentation of pitted dates by S. cerevisiae for the production of concentrated fructose syrups and ethanol. In Journal of Physics: Conference Series (Vol. 495, No. 1, p. 012034). IOP Publishing. 

King Saud University, Chemical Engineering Department, Saudi Arabia 

The metabolic stress response of tomato cell culture (Lycopersicum esculentum) to low oxygen studied using LAMBDA MINIFOR Bioreactor

Ampofo‐Asiama, J., Baiye, V. M. M., Hertog, M. L. A. T. M., Waelkens, E., Geeraerd, A. H., & Nicolai, B. M. (2014). The metabolic response of cultured tomato cells to low oxygen stress. Plant Biology, 16(3), 594-606. 

KU Leuven, Belgium; Flanders Centre of Postharvest Technology (VCBT), Leuven, Belgium.

LAMBDA MINIFOR bioreactor to grow the oral bacteria (Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis) under planktonic conditions

Blanc, V., Isabal, S., Sanchez, M. C., Llama‐Palacios, A., Herrera, D., Sanz, M., & León, R. (2014). Characterization and application of a flow system for in vitro multispecies oral biofilm formation. Journal of Periodontal Research, 49(3), 323-332. 

DENTAID S. L., Cerdanyola del Vallès, Spain; ETEP Research Group, University Complutense of Madrid, Spain. 

LAMBDA MINIFOR Bioreactor used for recombinant protein (Chemokines) expression in E. coli 

Kramp, B. (2014). Establishing the interaction between the CC chemokine ligand 5 and the receptors CCR1 and CCR (Doctoral dissertation, Aachen, Techn. Hochsch., Diss., 2013). (2024 Feb. 05)

RWTH Aachen, Germany.


Development of biofilm model (S. oralis, A. naeslundii, V. parvula, F. nucleatum, A. actinomycetemcomitans and P. gingivalis) in continuous flow in a LAMBDA MINIFOR 0.4L bioreactor.

Soto, I. S. (2013). Desarrollo del modelo de boca artificial en flujo continuo en el biorreactor Lambda Minifor. Universidad Complutense de Madrid Master en Ciencias Odontológicas. (2024 Feb. 05)

Recombinant expression of the Met-CCL5, protease resistant CXCL12 (S4V) and F1-CX3CL1 in E. coli using LAMBDA MINIFOR fermenter/bioreactor to study their role in Cardiovascular disease (CVD)

Projahn, D. (2013). Generation, function and therapeutic application of chemotactic cytokines in cardiovascular diseases (Doctoral dissertation, Aachen, Techn. Hochsch., Diss., 2013). (2024 Feb. 05)

RWTH Aachen, Germany.

Expression of Caf1 protein using Escherichia coli strain in LAMBDA MINIFOR fermentor to study mammalian cell adhesion, shape and number of focal adhesion

Machado Roque, A. I. (2013). Protein scaffolds for cell culture (Doctoral dissertation, Newcastle University). 

URI: (2024 Feb. 05)

Newcastle University, UK.

Controlled growth of Staphylococcus aureus under various concentrations of BAC (benzalkonium chloride) in LAMBDA MINIFOR fermentor

Cervinkova, D., Babak, V., Marosevic, D., Kubikova, I., & Jaglic, Z. (2013). The role of the qacA gene in mediating resistance to quaternary ammonium compounds. Microbial Drug Resistance, 19(3), 160-167.

Veterinary Research Institute, Brno, Czech Republic.


Effective production of Biobutanol from agricultural waste (giant hogweed, hay) using LAMBDA MINIFOR bench-top laboratory fermenter

Mezule, L., Tihomirova, K., Nescerecka, A., & Juhna, T. (2012). Biobutanol production from agricultural waste: A simple approach for pre-treatment and hydrolysis. Latvian Journal of Chemistry, 51(4), 407. 


Bioethanol production using Yeast (S. cerevisiae) in LAMBDA MINIFOR fermenter

Burešová, I., & Hřivna, L. (2011). Effect of wheat gluten proteins on bioethanol yield from grain. Applied Energy, 88(4), 1205-1210. 


Anaerobic fermentation of the glucose component in dates extract by yeast Saccharomyces cerevisiae

Gaily, M. H., Elhassan, B. M., Abasaeed, A. E., & Al-Zahrani, S. M. (2010). A direct process for the production of high fructose syrups from dates extracts. International Journal of Food Engineering, 6(3). 

King Saud University, Saudi Arabia; University of Khartoum, Sudan 

Study of the potential of tree tobacco stems (Nicotiana Glauca r. Grah.) as a bioethanol feedstock with the LAMBDA MINIFOR fermenter

Sánchez, F., Curt, M. D., Barreiro, M., Fernández, J., Agüera, J. M., Uceda, M., & Zaragoza, G. (2010). TREE TOBACCO (NICOTIANA GLAUCA R. GRAH.) STEMS AS A BIOETHANOL FEEDSTOCK. 18th European Biomass Conference and Exhibition, 3-7 May 2010, Lyon, France. (2024 Feb. 05)

Dpt. Producción Vegetal: Botánica y Protección Vegetal. Universidad Politécnica de Madrid (UPM), Madrid, Spain


Determination of the alcoholigenous potential of non-cellulosic carbohydrates from prickly pear cladodes by fermentation with the yeast Saccharomyces cerevisiae (commercial strains)

Sánchez, F., Curt, M. D., Fernández, J., Agüera, J. M., Uceda, M., & Zaragoza, G. (2009). Bioethanol production from prickly pear (Opuntia ficus-indica (L.) Mill.) cladodes. In Proc. 17th European Biomass Conference. Pub. ETA-Florence Renewable Energies & WIP-Renewable Energies. ISBN (pp. 978-88). (2024 Feb. 05)

Dpt. Producción Vegetal: Botánica y Protección Vegetal. Universidad Politécnica de Madrid (UPM), Madrid, Spain


Anaerobic expression using the LAMBDA MINIFOR

Park, M. O., Mizutani, T., & Jones, P. R. (2007). Glyceraldehyde-3-phosphate ferredoxin oxidoreductase from Methanococcus maripaludis. Journal of bacteriology, 189(20), 7281-7289. 

Research and Development Division, Fujirebio Incorporated, Japan.


Systems for High-Density Hybridoma Growth and High-yield mAb production in cell culture: Bench-top stirred tank bioreactors, 1-5 L (MINIFOR - LAMBDA Laboratory Instruments)

Howard, G.C., & Kaser, M.R. (Eds.). (2006). Making and Using Antibodies: A Practical Handbook (1st ed.). CRC Press.   



pH and temperature continuously recorded with the LAMBDA MINIFOR and SIAM software

Chaignon, P., Cortial, S., Guerineau, V., Adeline, M. T., Giannotti, C., Fan, G., & Ouazzani, J. (2005). Photochemical Reactivity of Trifluoromethyl Aromatic Amines: The Example of 3, 5‐diamino‐trifluoromethyl‐benzene (3, 5‐DABTF). Photochemistry and photobiology, 81(6), 1539-1543. 

Institut de Chimie des Substances Naturelles, C.N.R.S, France.


Bioreactors - An overview of the innovations implemented in MINIFOR bioreactors

Lehky, P. (2003) Bioreactors - New Solutions for Old Problems. International Congress on Bioreactor Technology, Tampere, Finland. (2024 Feb. 05)

Do you sell/ship to the USA?

Yes, we do supply our instruments directly with door-to-door delivery option by the parcel services to the USA.

What is the availability of the product?

We have the instruments in stock. We would just have to configure the instruments according to your requirements and perform quality control before shipping.

Is there a warranty?

We offer a 2 year warranty for MINIFOR fermentor / bioreactor and 5 year warranty for the PRECIFLOW & MULTIFLOW peristaltic pumps.

Does this fermentor work on both mammalian cells and yeast cells?

Yes, MINIFOR fermentor and bioreactor can be used for mammalian and yeast cell cultures (More information at

Is there flexibility in the top plate to add or remove probes?

Yes, MINIFOR has free ports in the headspace for the additional probes (sensors). Multiple ports and other effective solutions in the fermentation glass vess make the MINIFOR configuration equivalent to 16 to 22 classical ports (it is possible to increase the number of ports – custom made solution)

Is the equipment suitable for use in pure / mixed culture?

Yes, MINIFOR is suitable for pure as well as mixed culture. The stirrer is strong and can easily be adapted according to the types of cultures and working volumes.

Why is MINIFOR perfectly suitable for parallel processes?

Each unit stays independent as it is equipped with a control panel and display and at a single glance shows the parameter values. All parameters are regulated locally inside each fermenter-bioreactor unit.

This allows fast and precise parameter regulation and never having to worry about leaving a vessel unattended. Further advantage is that in case there are problems with one unit, the other units will still keep running.

How important is the slowdown in parameter regulation while running 12 bioreactors in parallel?

An important aspect to consider – which, however, does not play a role in the LAMBDA MINIFOR parallel system because each MINIFOR fermenter comes with its proper regulation unit that measures and controls all parameters locally. As a consequence the quality of the measurement and regulation is not affected by long transmission times and dead times in regulation.

How much space is required for the MINIFOR unit?

Footprint: approximately a sheet of paper
Dimensions: 22 cm x 38 cm x 40 cm (W x H x D)

Scale module for continuous cultures Vezi detalii
REDOX potential measurement [mV] Vezi detalii
LAMBDA MINI-4-GAS automatic 4-gas station for cell culture MINI-4-GAS Automatic gas-mix Vezi detalii
Automatic antifoam control Vezi detalii
FNet Programa de Control de la Fermentación FNet - Fermentor Control Software Vezi detalii
SIAM industrial fermentation software Vezi detalii
MINI-4-GAS software module Vezi detalii
OXYMETER O2 concentration measurement (0-25%) Vezi detalii
CARBOMETER CO2 concentration measurement (0-100%) Vezi detalii
METHAMETER CH4 concentration measurement (0-100%) Vezi detalii
Additional PRECIFLOW pump line PRECIFLOW pump 0-600 ml/h, reagent bottle with pipes, fittings, filter, tubing Vezi detalii
Additional MULTIFLOW pump line MULTIFLOW pump, reagent bottle with pipes, fittings, filter and tubing Vezi detalii