Biyoreaktör - Fermentör

Biyoreaktör - Fermentör

Yenilikler sayesinde düşük maliyetli, yüksek kaliteli bir fermenter üretilmiştir. LAMBDA MINIFOR, fermantasyon ve hücre kültürleri alanında yeni kavramlar geliştirmiştir:

LAMBDA MINIFOR tezgah üstü fermentör ve biyoreaktör

LAMBDA MINIFOR tezgah üstü laboratuar fermantörü ve biyoreaktörü, 35 ml’den 6 litreye kadar hacimlerde küçük bir laboratuar fermentörü inşa etme ihtiyacının bir sonucu olarak geliştirildi. Fermentasyonların uzun kişisel pratik deneyimlerine dayanarak, kullanımı kolay ve biyolojik kültürün bütün önemli parametrelerini ölçme ve kontrol etme kapasitesine sahip bir fermentör yaratmak istedik.

Fermantasyon tezgahı üzerinde minimum yer tutmak zorunda kaldı, ancak tüm parçalara iyi erişimi vardı. Birkaç fermantör, yan yana yerleştirildiğinde, kültür büyüme parametrelerinin optimizasyonu veya biyo-dönüşümlerin optimizasyonu için uygun olmalıdır.

Her fermentör, gelişmiş düzenleme ve kapsamlı veri işlemi için bağımsız olarak çalışabilir veya bir PC’ye bağlanabilir olmalıdır.

  • “Kolay sterilite” kavramı
  • Sıcaklık, pH, pO2 (çözünmüş oksijen (DO)), hava debisi parametrelerinin otomatik kontrolü
  • Tamamı camdan imal edilen, ağızları ve kapakları vidalı yeni kaplar kullanım esnekliğini arttırmıştır
  • Tek bir cihazda 35 ml ila 6 litre kültür hacimleri
  • Kademeli, hassas ve ekonomik kültür ısıtması için yeni kızılötesi radyatör (pahalı su banyolarına ihtiyaç duyulmaz)
  • Son derece kompakt, kullanışlı ve her taraftan rahatça ulaşılabilir
  • Hücre kültürlerinin yumuşak ve etkili şekilde karıştırılması için yeni “balık kuyruğu” karıştırıcı
  • Kesikli, beslemeli kesikli ve kesintisiz kültür işlemi
  • Hassas kütle akış ölçümü ile gaz akış kontrolü
  • Otomatik köpürme önleme kontrolü (opsiyonel)
  • Çok hızlı ve kolay kurma ve sökme
  • Yaygın şekilde kullanılan otoklavlarda sterile edilebilme
  • Kullanılan modern yüksek teknoloji ürünü malzemeler
  • Tek başına veya bilgisayarla kontrol edilebilme
  • FNet veya SIAM fermenter kontrol yazılımı (opsiyonel)
  • Paralel fermantasyon prosesleri için de uygundur
  • Garanti: 2 yıl

 

MINIFOR fermantör-biyoreaktörün maliyetini kaliteden ödün vermeden düşük tutmak için birkaç yeni fikir ve yenilik getirildi:

Paslanmaz çelik kapaklı, fermentörlü bir şişe yerine pahalıdır, dişli armatürlerle tüm cam kapları kullanırız. Hücre kültüründe uzun yıllar kullanılmaktadırlar ve kusursuz sterilliği sürdürdüğü kanıtlanmıştır. Bu konsept sayesinde MINIFOR fermantör-biyoreaktör mümkün olan en kısa sürede kurulur.

Pahalı bir motor ve manyetik kuplaj gerektiren geleneksel bir pervane karıştırıcı yerine, yeni bir yukarı-aşağı karıştırma işlemi başlattık. Ucuz bir membranla birlikte bir motor, sterilliği mükemmel bir şekilde garanti eder ve bir vorteks oluşmadan verimli bir şekilde karıştırma sağlar (hiçbir bölme gerekmez). Aynı zamanda bu tür karıştırma hücreler üzerinde daha naziktir ve daha az köpük üretir. Yeni biomimikasyon “balık kuyruğu” karıştırma diskleri, kenarları kesmeden maksimum karıştırma etkinliği sunar.

Kültür, parabolik bir radyatörde üretilen ısı radyasyonu ile fermantasyon kabının altına yerleştirilen altın reflektör ile ısıtılır. Isı, güneş ısıtma suyuna benzer şekilde kültüre hafifçe emilir. Herhangi bir hacimde kültürün aşırı ısınması yoktur ve termostatik banyolar içeren pahalı çift duvarlı kaplar elimine edilir. Aynı zamanda boru ve kablolar yok olur ve fermentörü daha az karmaşık hale getirir.

Mümkün olduğunca pahallı ekipman parçaları yeni yüksek performanslı plastiklerle değiştirildi.

Modern mikroişlemcileri kullanarak tüm elektronikleri cihazın ön kısmına yerleştirmek mümkündür. Bu, fermentörü inanılmaz derecede kompakt yapar ve muhafaza kulesini ortadan kaldırır. Küçük boyutuna rağmen, MINIFOR’un temel konfigürasyonunda altı parametre ölçülmekte ve kontrol edilmektedir.

Power: Universal power supply for mains 100-245 V AC/50-60Hz, 560W, CE conform
Dimensions: 22 x 40 x 38 cm (W x D x H)
Display: LCD 4 x 40 digits with backlight illumination
Fermentor vessel: Pyrex glass with 6 to 8 side necks; 0.3, 0.4, 1, 3, 7 liter vessels
Temperature control: High efficiency 150 W infrared (IR) radiation heat source with gilded parabolic reflector
Regulation: from 5°C over RT to 70°C
Measurement: from 0 to 99.9°C in 0.1°C steps
Precision: +/- 0.2°C (0 to 60°C)
Sensor: Pt 100 incorporated in the glass electrode of the pH probe
pH control: sterilisable pH electrode pH 0-14 with automatic temperature correction, two-point semiautomatic calibration and Variopin connector
Resolution: 0.01 pH unit
Precision: +/- 0.02 pH unit
pO2 control: sterilisable Clark type oxygen sensor with fast response, automatic temperature correction, two-point semiautomatic calibration, dissolved oxygen (DO) control through regulation of the airflow rate
Range: 0 to 25 mg oxygen/ l, in 0.1 mg/l steps
Air flow: 0 to 5 l/min in 0.01 l/min steps, measured by precise mass flow meter, linearity +/- 3%, reproducibility +/- 0.5%
Control: proportional valve controlled by microprocessor
For supplied air pressure: 0.05 – 0.2 MPa (0.5 - 2 atm)
Agitation: 50 W Vibromixer 0 to 20 Hz (0 to 1200 rpm) in 0.1 Hz steps (6 rpm) with 1 or more stirring discs; Sterility similar to magnetic coupling
Selectable parameter 'X': an additional parameter can be controlled by the instrument (foaming control, weight (for continuous cultures), pCO2, redox potential, conductivity, optical density, etc.); with standard 0-10V or 0-20mA output
Ports / side necks: One large quadruple sampling or additions port with four needles with LAMBDA PEEK double-seal connections, used for sampling, inoculation, antifoam, feeds, harvest, addition of correction solutions etc., additional double ports are available.
Pumps: up to 4 independent pumps (PRECIFLOW, MULTIFLOW, HIFLOW or MAXIFLOW) with speed variation from 0 to 100 % can be used with MINIFOR lab fermenter-bioreactor
Gas flow control: In addition to pumps, several electronic flow controllers with flow rate ranges of 0-5 l/min (MASSFLOW 5000) or 0-500 ml/min (MASSFLOW 500) can be used for the controlled addition of gases (e.g. N2, O2, air, CO2) in cell cultures; freely configurable gas station module
Working temperature: 0 – 40 °C
Working humidity: 0 - 90 % RH, not condensing
Weight: 7.5 kg
PC control: complete PC control and data processing using the fermentation software FNet (for up to 6 MINIFOR fermenters) or SIAM (for an even higher number of instruments)

2018: MINIFOR lab scale bioreactor used for production of bioethanol from lignocellulosic biodegradable municipal solid waste (BMSW) under optimized conditions

Hayder, Nadhim H., Hussain M. Flayeh, and Ali W. Ahmed. "Optimization of Bioethanol Production from Biodegradable Municipal Solid Waste using Response Surface Methodology (RSM)." Journal of Engineering and Sustainable Development Vol 22, no. 01 (2018).

Baghdad University, Biotechnology Department and  Environmental Engineering Department, Baghdad, Iraq.

Keywords:  Biodegradable municipal solid waste; Bioethanol; Fermentation; Bioreactor


2017: Efficient ethanol production from whey permeate (WP) and concentrated permeate (CWP) with engineered E. coli in pH-controlled bioreactor MINIFOR
Pasotti, Lorenzo, Susanna Zucca, Michela Casanova, Giuseppina Micoli, Maria Gabriella Cusella De Angelis, and Paolo Magni. "Fermentation of lactose to ethanol in cheese whey permeate and concentrated permeate by engineered Escherichia coli." BMC biotechnology 17, no. 1 (2017): 48.
University of Pavia, Laboratory of Bioinformatics, Mathematical Modelling and Synthetic Biology, Department of Electrical, Computer and Biomedical Engineering, Pavia, Italy; University of Pavia, Centre for Health Technologies, Pavia, Italy.
Keywords: Ethanol; Lactose; Fermentation; Escherichia coli; Whey permeate


2017: The effect of different temperatures on sugar starvation in cells isolated from fresh leafy vegetables was studied in MINIFOR bioreactor
Mbong, Victor Baiye Mfortaw, Jerry Ampofo-Asiama, Maarten LATM Hertog, Annemie H. Geeraerd, and Bart M. Nicolai. "The effect of temperature on the metabolic response of lamb’s lettuce (Valerianella locusta,(L), Laterr.) cells to sugar starvation." Postharvest Biology and Technology 125 (2017): 1-12.
KU Leuven, Division of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST), Leuven, Belgium; Flanders Centre of Postharvest Technology (VCBT), Leuven, Belgium.
Keywords: Isolated lamb’s lettuce cells; Sugar starvation; Temperature; Metabolome; 13C label


2017: LAMBDA MINIFOR bioreactor for the production of CB.Hep-1 mAb using mouse hybridoma cell culture in protein-free media
Valdés R, Aragón H, González M, Hernández D, Geada D, Goitizolo D et al. Mouse hybridoma cell culture in a protein-free medium using a bio-mimicking fish-tail disc stirred bioreactor. BioProcess J, 2017; 16(1): 51–64.
CIGB, Havana, Cuba.


2016: Robust cellulosic ethanol production from sugarcane bagasse with Saccharomyces cerevisiae ATCC 20602 in LAMBDA MINIFOR laboratory bioreactor under aerobic and anaerobic conditions with controlled redox potential measurement
Jabasingh, S. Anuradha, et al. "Catalytic conversion of sugarcane bagasse to cellulosic ethanol: TiO2 coupled nanocellulose as an effective hydrolysis enhancer." Carbohydrate polymers 136 (2016): 700-709.
Addis Ababa Institute of Technology, Ethiopia; Sathyabama University, India.
Keywords: Cellulosic ethanol; bagasse; Titanium dioxide; Nanocellulose; Cellulase Saccharomyces cerevisiae


2015: S. pyogenes Cas9 protein expressed using a 3L computer-controlled MINIFOR bioreactor in batch medium followed by exponential feeding
Ménoret, Séverine, et al. "Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins." Scientific reports 5 (2015): 14410.
INSERM UMR 1064-ITUN; CNRS UMS3556 Nantes; CNRS UMR7196; Sorbonne Universities; University Pierre & Marie Curie; France.


2015: Fermentation of engineered microorganism in laboratory scale bioreactor MINIFOR for efficient conversion of lactose-to-ethanol
Pasotti, Lorenzo, et al. "Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology." Engineering in Medicine and Biology Society (EMBC), 2015 37th Annual International Conference of the IEEE. IEEE, 2015.
University of Pavia, Department of Electrical, Computer & Biomedical Engineering and Interdepartmental Research Centre for Tissue Engineering, Italy
Keywords: lactose-to-ethanol conversion; microorganism optimization; synthetic biology; whey protein; permeate; pollutant waste disposal; genetic optimization; green energy production; biofuel production; cheese production process; dairy waste; biocatalyst


2015: Six-species flow cell biofilm model was developed by culturing bacteria in LAMBDA MINIFOR Bioreactor to evaluate the biofilm development under flow and shear conditions
Salli, Krista M., and Arthur C. Ouwehand. "The use of in vitro model systems to study dental biofilms associated with caries: a short review." Journal of oral microbiology 7 (2015).
DuPont Nutrition and Health, Kantvik Active Nutrition, Finland.
Keywords: dental caries, batch culture, continuous culture, artificial mouth, flow cell, microcosm 


2015: Quantification of ribosomal proteins (RPs) from Yeast cells cultured in MINIFOR bioreactor and mouse embryonic stem cells (ESC) to study the core RPs stoichiometry
Slavov, Nikolai, et al. "Differential stoichiometry among core ribosomal proteins." Cell reports 13.5 (2015): 865-873.
Harvard University, USA; Broad Institute of MIT and Harvard, USA and Hubrecht Institute, Netherlands.
Keywords: Budding Yeast cells, Embryonic stem cells (ESC), Ribosomal Protein, RP, ribosomes, mRNA, mass-spectrometry, posttranslational modification, PTM


2014: Cultivation of microalgae (Chlorella vulgaris Beyerinck) in laboratory bioreactor MINIFOR
Heitur, Heiko. Mikrovetika Chlorella vulgaris Beyerincki kasvatamine CO2 sidumise eesmärgil. Diss. 2014.
Eesti Maaülikool (Estonian University of Life Sciences), Estonia.
Keywords: CO2, microalgae, growth rate, photobioreactor


2014: Growing yeast cultures (DBY12007) in the MINIFOR fermenter at steady state to study the aerobic glycolysis and energy flux
Slavov, Nikolai, et al. "Constant growth rate can be supported by decreasing energy flux and increasing aerobic glycolysis." Cell reports 7.3 (2014): 705-714.
Massachusetts Institute of Technology, USA; Harvard University, USA; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, Netherlands and Princeton University, USA.
Keywords: Yeast, aerobic glycolysis, exponential growth, O2 consumption, CO2 production, amino acids, mRNAs, proteins, posttranslational modifications, stress sensitivity, respiratory quotient (RQ)


2014: Selective and non-selective batch fermentation of date extract using Saccharomyces cerevisiae (commercial strain used in bakeries (wild strain), glucose selective strains ATCC 36858 and ATCC 36859) studied in LAMBDA MINIFOR fermentor
Putra, Meilana Dharma, et al. "Selective fermentation of pitted dates by S. cerevisiae for the production of concentrated fructose syrups and ethanol." Journal of Physics: Conference Series. Vol. 495. No. 1. IOP Publishing, 2014.
King Saud University, Chemical Engineering Department, Saudi Arabia 
Keywords: Selective, non-selective, fermentation, yeast, S. cerevisiae, fructose, ethanol, date, HPLC, kinetic profile, batch


2014: The metabolic stress response of tomato cell culture (Lycopersicum esculentum) to low oxygen studied using LAMBDA MINIFOR Bioreactor
Ampofo‐Asiama, Jerry, et al. "The metabolic response of cultured tomato cells to low oxygen stress." Plant Biology 16.3 (2014): 594-606.
KU Leuven, Belgium; Flanders Centre of Postharvest Technology (VCBT), Leuven, Belgium;
Keywords: 3C label; cell culture; low O2 stress; Lycopersicum esculentum; metabolome


2014: LAMBDA MINIFOR bioreactor to grow the oral bacteria (Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis) under planktonic conditions
Blanc, V., et al. "Characterization and application of a flow system for in vitro multispecies oral biofilm formation." Journal of periodontal research 49.3 (2014): 323-332.
DENTAID S. L., Cerdanyola del Vallès, Spain; ETEP Research Group, University Complutense of Madrid, Spain;
Keywords: biofilm model; chlorhexidine; confocal laser scanning microscopy; oral bacteria


2013: Recombinant expression of the Met-CCL5, protease resistant CXCL12 (S4V) and F1-CX3CL1 in E. coli using MINIFOR fermenter/bioreactor to study their role in Cardiovascular disease (CVD)
Projahn, Delia, and Christian Weber. Generation, function and therapeutic application of chemotactic cytokines in cardiovascular diseases. Diss. Hochschulbibliothek der Rheinisch-Westfälischen Technischen Hochschule Aachen, 2013.
RWTH Aachen, Germany.


2013: Expression of Caf1 protein using Escherichia coli strain in MINIFOR fermentor to study mammalian cell adhesion, shape and number of focal adhesion
Machado Roque, Ana Isabel. "Protein scaffolds for cell culture." (2013).
Newcastle University, UK.


2013: LAMBDA MINIFOR Bioreactor used for recombinant protein (Chemokines) expression in E. coli
Kramp, Birgit, and Robert Ryan Koenen. Establishing the interaction between the CC chemokine ligand 5 and the receptors CCR1 and CCR. Diss. Hochschulbibliothek der Rheinisch-Westfälischen Technischen Hochschule Aachen, 2013.
RWTH Aachen, Germany.


2013: Systems for High-Density Hybridoma Growth and High-yield mAb production in cell culture: Bench-top stirred tank bioreactors, 1-5 L (MINIFOR - LAMBDA Laboratory Instruments)
Kase, Matthew R., ed. Making and using antibodies: a practical handbook. CRC press, 2013.


2013: Controlled growth of Staphylococcus aureus under various concentrations of BAC (benzalkonium chloride) in MINIFOR fermentor
Cervinkova, Dana, et al. "The role of the qacA gene in mediating resistance to quaternary ammonium compounds." Microbial Drug Resistance 19.3 (2013): 160-167.
Veterinary Research Institute, Brno, Czech Republic.
Keywords: Staphylococcus aureus, benzalkonium chloride (BAC), exponential phase, expression, real-time PCR, culture, concentration


2012: Effective production of Biobutanol from agricultural waste (giant hogweed, hay) using MINIFOR bench-top laboratory fermenter
Mezule, L., et al. "Biobutanol production from agricultural waste: A simple approach for pre-treatment and hydrolysis." Latvian Journal of Chemistry 51.4 (2012): 407-414.
Riga Technical University, Latvia 
Keywords: biofuel, biobutanol, agricultural waste, hydrolysis


2012: MINIFOR Bioreactor for stem cells
Shayan, Niloufar, et al. "A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cells." Biotechnology letters 34.11 (2012): 2125-2131.
Department of Regenerative Medicine, Cell Research Center, Royan Institute for Stem Cell Biology and Technology, Royan Cord Blood Bank, Tehran, Iran; Department of Stem Cells and Developmental Biology, Cell Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran 
Keywords: Static culture; Suspension bioreactor; Umbilical cord blood; Vertical mixing


2011: Bioethanol production using Yeast (S. cerevisiae) in LAMBDA MINIFOR fermenter
Burešová, Iva, and Luděk Hřivna. "Effect of wheat gluten proteins on bioethanol yield from grain." Applied Energy 88.4 (2011): 1205-1210.
Agrotest Fyto, Ltd., Kroměříž, Czech Republic; Mendel University in Brno, Czech Republic 
Keywords: Bioethanol; Triticale; Wheat; Gluten; Protein


2010: Anaerobic fermentation of the glucose component in dates extract by yeast Saccharomyces cerevisiae
Gaily, Mohamed H., et al. "A Direct Process for the Production of High Fructose Syrups from Dates Extracts." International Journal of Food Engineering 6.3 (2010): 12.
King Saud University, Saudi Arabia; University of Khartoum, Sudan 
Keywords: dates, fructose, glucose, ethanol, fermentation, S. Cerevisiae, yeast, mesophilic, batch


2007: Anaerobic expression using the LAMBDA MINIFOR
Park, Myong-Ok, Taeko Mizutani, and Patrik R. Jones. "Glyceraldehyde-3-phosphate ferredoxin oxidoreductase from Methanococcus maripaludis." Journal of bacteriology 189.20 (2007): 7281-7289.
Research and Development Division, Fujirebio Incorporated, Japan.


2005: pH and temperature continuously recorded with the LAMBDA MINIFOR and SIAM software
Chaignon, Philippe, et al. "Photochemical reactivity of trifluoromethyl aromatic amines: the example of 3, 5-diamino-trifluoromethyl-benzene (3, 5-DABTF)." Photochemistry and photobiology 81.6 (2005): 1539-1543.
Institut de Chimie des Substances Naturelles, C.N.R.S, France.


 

Do you sell/ship to the USA?

Yes, we do supply our instruments directly with door-to-door delivery option by the parcel services to the USA.


What is the availability of the product?

We have the instruments in stock. We would just have to configure the instruments according to your requirements and perform quality control before shipping. 


 Is there a warranty?

We offer a 2 year warranty for MINIFOR fermentor / bioreactor and 5 year warranty for the PRECIFLOW & MULTIFLOW peristaltic pumps.


Does this fermentor work on both mammalian cells and yeast cells?

Yes, MINIFOR fermentor and bioreactor can be used for mammalian and yeast cell cultures (More information at www.fermentor.net/applications).


Is there flexibility in the top plate to add or remove probes?

Yes, MINIFOR has free ports in the headspace for the additional probes (sensors). Multiple ports and other effective solutions in the fermentation glass vess make the MINIFOR configuration equivalent to 16 to 22 classical ports (it is possible to increase the number of ports – custom made solution)


Is the equipment suitable for use in pure / mixed culture?

Yes, MINIFOR is suitable for pure as well as mixed culture. The stirrer is strong and can easily be adapted according to the types of cultures and working volumes.


Why is MINIFOR perfectly suitable for parallel processes?

Each unit stays independent as it is equipped with a control panel and display and at a single glance shows the parameter values. All parameters are regulated locally inside each fermenter-bioreactor unit.

This allows fast and precise parameter regulation and never having to worry about leaving a vessel unattended. Further advantage is that in case there are problems with one unit, the other units will still keep running.


How important is the slowdown in parameter regulation while running 12 bioreactors in parallel?

An important aspect to consider – which, however, does not play a role in the LAMBDA MINIFOR parallel system because each MINIFOR fermenter comes with its proper regulation unit that measures and controls all parameters locally. As a consequence the quality of the measurement and regulation is not affected by long transmission times and dead times in regulation.


How much space is required for the MINIFOR unit?

Footprint: approximately a sheet of paper
Dimensions: 22 cm x 38 cm x 40 cm (W x H x D)

Scale module for continuous cultures Detaylı bilgi talebi
REDOX potential measurement [mV] Detaylı bilgi talebi
LAMBDA MINI-4-GAS automatic 4-gas station for cell culture MINI-4-GAS Automatic gas-mix Detaylı bilgi talebi
Automatic antifoam control Detaylı bilgi talebi
FNet Programa de Control de la Fermentación FNet - Fermentor Control Software Detaylı bilgi talebi
SIAM industrial fermentation software Detaylı bilgi talebi
MINI-4-GAS software module Detaylı bilgi talebi
OXYMETER O2 concentration measurement (0-25%) Detaylı bilgi talebi
CARBOMETER CO2 concentration measurement (0-100%) Detaylı bilgi talebi
METHAMETER CH4 concentration measurement (0-100%) Detaylı bilgi talebi
Additional PRECIFLOW pump line PRECIFLOW pump 0-600 ml/h, reagent bottle with pipes, fittings, filter, tubing Detaylı bilgi talebi
Additional MULTIFLOW pump line MULTIFLOW pump, reagent bottle with pipes, fittings, filter and tubing Detaylı bilgi talebi