2024

Simultaneous Saccharification and Fermentation (SSF): The LAMBDA MINIFOR bioreactor was used for both the fermentation of pretreated solid biomass (10 %) in the presence of cellulase, β-glucosidase and inoculated with Lactobacillus rhamnosus (1.7 L, 37 °C, pH=5.5, 72 h), as well as for the subsequent fermentation of hydrolysates with Bacillus megaterium (1.7 L, 35 °C, 48 h).

Senila, L., Kovacs, E., Resz, M. A., Senila, M., Becze, A., & Roman, C. (2024). Life Cycle Assessment (LCA) of Bioplastics Production from Lignocellulosic Waste (Study Case: PLA and PHB). Polymers 2024, 16, 3330. 

https://doi.org/10.3390/polym16233330

Anaerobic batch cultivation of C. butyricum in a LAMBDA MINIFOR 3L fermenter, flushed with argon prior to fermentation: pH 5.6 (controlled with 1M NaOH), 37°C and 6000 rpm. Gas production was measured by the water displacement method, displaced water volume monitored by a camera. Liquid and gas samples were collected at intervals.

Pištěková, H., Dusankova, M., Sopik, T., Klaban, J., Dostalkova, J., Moučka, R., & Sedlarik, V. Optimization of the Dark Fermentation Technique for Hydrogen Production Through Supplementation with Ascorbic Acid And/Or L-Cysteine by Clostridium Butyricum Ccdbc 11. Or L-Cysteine by Clostridium Butyricum Ccdbc, 11.
https://dx.doi.org/10.2139/ssrn.4973469 

Alcoholic fermentations tested under microvinification conditions and in a LAMBDA MINIFOR bioreactor with controlled parameters (T = 20 °C, agitation frequency = 2 Hz) and standard conditions (T = 12 - 14 °C).

Filimon, G. (2024). Monitorizarea fermentaţiei alcoolice a musturilor obţinute din soiuri de struguri rizogene. Universitatea Tehnică a Moldovei. Technical Scientific Conference of Undergraduate, Master and PhD Students: Chişinău, 27-29 martie 2024. Vol. 2.
URI: http://repository.utm.md/handle/5014/28059 

Biodegradation: Treatment of marine samples with the addition of “Diesel-B5” (10 %, 15 %, 20 %) as a C-source in aerated and stirred LAMBDA MINIFOR bioreactors (Pseudomonas sp.; pH 6.5; incubation temperatures 25 °C, 35 °C and 45 °C).

Peña Ventura, J. C. (2024). Determinación del ratio de biodegradación de hidrocarburos totales de petróleo empleando Pseudomonas sp. en agua de mar contaminada con diésel-B5. Universidad Nacional de Trujillo, Unidad de Postgrado en Ingeniería Química, Tesis de maestro en ingeniería química ambiental.
URI: https://hdl.handle.net/20.500.14414/22166 

"The mixed inoculum was inoculated into the reaction chamber of the LAMBDA MINIFOR bioreactor, which was maintained at 37 °C under anaerobic conditions."

Khan, S. N., Ribeiro‐Vidal, H., Virto, L., Bravo, E., Nuevo, P., Koldsland, O. C., Hjortsjö, C. & Sanz, M. (2024). The Decontamination Effect of an Oscillating Chitosan Brush Compared With an Ultrasonic PEEK‐Tip: An In Vitro Study Using a Dynamic Biofilm Model. Clinical Oral Implants Research.
https://doi.org/10.1111/clr.14360 

The batch co-culture fermentation of date palm syrup with S. cerevisiae and P. stipitis ATCC 58785 was carried out in a LAMBDA MINIFOR 1L Advanced Kit stirred tank fermenter.

Shalsh, F. J., Gatte, E. H., Alsultan, I. I., Tariq, F. Z., Jassim, S. M., Saleh, M. T., & Alrazzaq, N. A. (2024). Bioplastic Compounds of Succinic Acid from Agriculture Waste; Date Palm Syrup And Date Palm Fronds. Nepal Journal of Biotechnology, 12(1), 32-39.
https://doi.org/10.54796/njb.v12i1.310 

In vitro development of a dynamic multispecies biofilm model on implant surfaces: Planktonic growth of a mixture of six different bacterial strains inside the LAMBDA MINIFOR bioreactor (12 h), followed by continuous culture (flow rate of 30 mL/h) through 2 Robbins devices containing the sterile implants (37 °C, anaerobic, 72 h).

Khan, S. (2024). Efficacy of Mechanical Decontamination Strategies in the Treatment of Peri-implantitis. Doctoral thesis, University of Oslo.
URI: https://hdl.handle.net/10852/111365 (2024 August 07)

In vitro dynamic multispecies biofilm model: During the entire incubation process (biofilms growing on implant surfaces), the LAMBDA MINIFOR bioreactor maintains the culture medium at 37 °C, pH 7.2 and an anaerobic atmosphere (10% H₂, 10% CO₂ and N₂ balance).

Bravo, E., Arce, M., Ribeiro-Vidal, H., Herrera, D., & Sanz, M. (2024). The Impact of Candida albicans in the Development, Kinetics, Structure, and Cell Viability of Biofilms on Implant Surfaces—An In Vitro Study with a Validated Multispecies Biofilm Model. International Journal of Molecular Sciences, 25(6), 3277.
https://doi.org/10.3390/ijms25063277 

Submerged batch (consortia) and fed-batch (L. coryniformis) fermentations in LAMBDA MINIFOR stirred-tank bioreactor and SIAM software: 72 h, final working volumes = 200 ml, T = 37 ± 0.5 °C, stirring 0.5 Hz, sparing CO₂ at 0.04 L/min, pH 6.5 ± 0.5 using 1 M NaOH and 1 M H₂SO₄ for automatic pH adjustment; samples were taken in regular intervals.

Buljubašić, E., Bambace, M. F., Christensen, M. H. L., Ng, K. S., Huertas‐Díaz, L., Sundekilde, U., Marietou, A. & Schwab, C. (2024). Novel Lactobacillaceae strains and consortia to produce propionate‐containing fermentates as biopreservatives. Microbial Biotechnology, 17(2), e14392.
https://doi.org/10.1111/1751-7915.14392

The in vitro multispecies dynamic biofilm model was used which has been validated on biofilms growing on implant surfaces: LAMBDA MINIFOR bioreactor maintains the protein-enriched BHI medium under stable conditions: 37 °C, pH 7.2 and an anaerobic atmosphere by directly pumping an anaerobic gas mixture (10% H₂, 10% CO₂ and equilibrium N₂) keeping the pressure constant during the whole incubation process

Bravo, E., Arce, M., Ribeiro-Vidal, H., Herrera, D., & Sanz, M. (2024). The Impact of Candida albicans in the Development, Kinetics, Structure, and Cell Viability of Biofilms on Implant Surfaces. An In Vitro Study with a Validated Multispecies Biofilm Model.
https://doi.org/10.20944/preprints202402.1137.v1 

Simulated human colon model LAMBDA MINIFOR with basal nutrient medium (37 °C, pH 6.8) and faecal slurries

Pusuntisumpun, N., Tunsagool, P., Nitisinprasert, S., & Nakphaichit, M. (2024). Impacts of combining Limosilactobacillus reuteri KUB‐AC5 and Limosilactobacillus fermentum KUB‐D18 on overweight gut microbiota using a simulated human colon model. International Journal of Food Science & Technology.


https://doi.org/10.1111/ijfs.16941

400 ml isolated tomato cells in a LAMBDA MINIFOR bioreactor (20 °C, pH 5.8) got sparged (10 L/h) to achieve varied O2 concentrations (21 kPa, 5 kPa, and 0 kPa) for the study of postharvest losses of fruits and vegetable during controlled atmosphere storage as a result of low O2 stress.

Mahomud, M. S., Islam, M., & Roy, J. (2024). Effect of low oxygen stress on the metabolic responses of tomato fruit cells. Heliyon, e24566.

https://doi.org/10.1016/j.heliyon.2024.e24566

Simulation of in-vitro gut model with LAMBDA MINIFOR 0.3L: Batch fermentations under anaerobic conditions (37 °C, pH 6.8–6.9, 24 h) of human fecal slurry (1% (v/v)) to evaluate the effect of Triphala extracts (1 mg/mL) co-fermentation on microbiota and metabolic changes.

Kwandee, P., Somnuk, S., Wanikorn, B., Nakphaichit, M. & Tunsagool, P. (2023). Efficacy of Triphala extracts on the changes of obese fecal microbiome and metabolome in the human gut model. Journal of Traditional and Complementary Medicine, Volume 13, Issue 2, 2023, Pages 207-217, ISSN 2225-4110.

Milk was pasteurized at 70 °C for 30 min in LAMBDA MINIFOR fermenters
/
LAMBDA MINIFOR fermenters: Temperature effect tests on growth of yeast Kazachstania unispora (initial ~10E6 CFU/ml) in milk (800 ml, ~6 % lactose): from 5 °C to 40 °C (5, 10, 15, 20, 25, 27, 30, 32, 35, 37, and 40 °C) at pH 5.6 (automatic adjustment with 2 M NaOH) and 240 rpm, until stationary phase was reached (inline near-infrared turbidity sensor Optek FC20- ASD10-N)
/
LAMBDA MINIFOR fermenters for coculture experiments at 25 °C of Lacticaseibacillus casei and Kazachstania unispora in modfied MRS media as well as in mare milk (initial: ~10E6 CFU/ml, pH = 6.8)
/
LAMBDA MINIFOR fermenters for coculture experiments at 30 °C of Lactobacillus kefiri and Kazachstania unispora in modfied MRS media as well as in mare milk (initial: ~10E6 CFU/ml, pH = 6.8)

Kondybayev, A., Achir, N., Mestres, C., Collombel, I., Strub, C., Grabulos, J., Akhmetsadykov, N., Aubakirova, A., Kamidinkyzy, U., Ghanmi, W. & Konuspayeva, G. (2023). Growth Kinetics of Kazachstania unispora and Its Interaction with Lactic Acid Bacteria during Qymyz Production. Fermentation 2023, 9, 101.

https://doi.org/10.3390/fermentation9020101 

2022:

LAMBDA MINIFOR bioreactor and fermentor for schooling & university 

Lactic acid bacteria growth experiments: Effect of temperature on Lacticaseibacillus casei and Lactobacillus kefiri.

The continuous culture was performed in a Lambda Photobioreactor (PBR). White light from the LAMBDA LUMO module was calibrated to umolm−2 m−1. For evaporation control and continuous culture mode, the total weight of the reactor setup was kept constant using the built-in LAMBDA reactor mass control module and automatic addition of fresh culture medium through the feed pump. Continuous culture was performed by setting the waste pump to a fixed speed.

Behle, A., Dietsch, M., Goldschmidt, L., Murugathas, W., Berwanger, L.C., Burmester, J., Yao, L., Brandt, D., Busche, T., Kalinowski, J., Hudson, E.P., Ebenhöh, O., Axmann, I.M. & Machné, R. (2022). Manipulation of topoisomerase expression inhibits cell division but not growth and reveals a distinctive promoter structure in Synechocystis. Nucleic Acids Research, Volume 50, Issue 22, 9 December 2022, Pages 12790–12808.

https://doi.org/10.1093/nar/gkac1132 

Biocatalytic resolution of lupanine racemate in industrial wastewater by Pseudomonas putida LPK411 using a lab‐scale bioreactor LAMBDA MINIFOR 0.4L under batch operation.

Parmaki, S., Esteves, T., Gonçalves, J.M.J. Catenacci, A., Malpei, F., Ferreira, F.C., Afonso C.A.M & Koutinas, M. (2022). Selective microbial resolution of lupanine racemate: Bioprocess development and the impact of carbon catabolite repression on industrial wastewater valorisation. Biomass Conv. Bioref. (2022).

https://doi.org/10.1007/s13399-022-03383-3 

For releasing bioactive peptides, diluted Kiwicha protein isolate (KPI) from Amaranthus caudatus L. seed flour was subjected to enzymatic hydrolysis with Bioprotease LA-660 using a LAMBDA MINIFOR bioreactor for continuous stirring, pH- and temperature control (pH 8; 50 °C).

Martinez-Lopez, A., Rivero-Pino, F., Villanueva, A., Toscano, R., Grao-Cruces, E., Marquez-Paradas, E., Martin, M.E., Montserrat-de la Paz, S. & Millan-Linaresa, M.C. (2022). Kiwicha (Amaranthus caudatus L.) protein hydrolysates reduce intestinal inflammation by modulating the NLRP3 inflammasome pathway. Food & Function 2022 Oct 21.

https://doi.org/10.1039/D2FO02177C 

Escherichia coli (E. coli; E44Δ) mutant strain for production of large quantity of outer membrane vesicles (OMVs) in a LAMBDA MINIFOR 7L fermenter. 

Allahghadry, T., Bojesen, A.M., Whitehead, B.J. and Antenucci, F. (2022). Clarification of large-volume bacterial cultures using a centrifuge-free protocol. J Appl Microbiol. Accepted Author Manuscript. 

https://doi.org/10.1111/jam.15608 

2021:

Experiments on liquid phase (hemicelluloses hydrolysate) for xylitol production: The fermentation of 250 ml of detoxified hydrolysate was conducted in a 1L fermenter (LAMBDA MINIFOR bench-top-laboratory-fermenter) and pH adjustment (pH 5.0) at aerobic conditions at 30 °C for 60 hr. 

Shalsh, D., Nagimm, D., Alrheem, M.A. & Alrheem, S.A. (2021). Batch fermentation and Simultaneous Saccharification and Fermentation (SSF) processes by Meyerozyma Guilliermondii Strain F22 and Saccharomyces cerecvisae for xylitol and bioethanol co-production. Al-Qadisiyah Journal of Pure Science, 26(4), 80–94.

https://doi.org/10.29350/qjps.2021.26.4.1347 

The growth, glucose consumption and ethanol production of Saccharomyces cerevisiae LM strain in synthetic broth were modeled for the most important intrinsic...One liter Lambda Minifor fermenters equipped with a cold water condenser on air exit pipes (LAMBDA Instruments GmbH, Baar- Switzerland), were used.

Kouamé, C., Loiseau, G., Grabulos, J., Boulanger, R. & Mestres, C. (2021). Development of a model for the alcoholic fermentation of cocoa beans by a Saccharomyces cerevisiae strain. International Journal of Food Microbiology, Volume 337, 2021, 108917, ISSN 0168-1605.

https://doi.org/10.1016/j.ijfoodmicro.2020.108917 

Continuous culture of cyanobacteria Synechocystis sp. PCC 6803 in a LAMBDA MINIFOR 1L PBR photo-bioreactor. 

Behle, A., Dietsch, M., Goldschmidt, L., Murugathas, W., Brandt, D., Busche, T., Kalinowski, J., Ebenhöh, O., Axmann, I. M. & Machné, R. (2021) Uncoupling of the Diurnal Growth Program by Artificial Genome Relaxation in Synechocystis sp. PCC 6803. bioRxiv 2021.07.26.453758.

https://doi.org/10.1101/2021.07.26.453758 

The hydrolysis of kiwicha protein isolate (KPI) is performed under continuous stirring, using a LAMBDA MINIFOR fermenter-bioreactor, at controlled conditions of pH and temperature: Bioprotease LA-660 is added at a ratio enzyme/substrate = 0.3 AU/g protein (pH 8) for 5, 10, 15, 30, and 60 min.

Paz, S. M. D. L., Martinez-Lopez, A., Villanueva-Lazo, A., Pedroche, J., Millan, F., & Millan-Linares, M. C. (2021). Identification and characterization of novel antioxidant protein hydrolysates from kiwicha (Amaranthus caudatus L.). Antioxidants, 10(5), 645.

https://doi.org/10.3390/antiox10050645

The biological transformation of white sorghum biomass was performed under operating conditions similar to the MixAlco process. MixAlco batch fermentation process were performed in the LAMBDA MINIFOR bioreactor.

Shalsh, F.J., Alrazzaq, N.A., Nagimm D.K., Alrheem, M.A., Alrheem S.A. & Abd-alalah, K. (2021). Bioconversion of white sorghum biomass using MixAlco fermentation process. DYSONA – Applied Science. 2021(2), 21-27. ISSN 2708-6283.

https://doi.org/10.30493/DAS.2021.248966

2020:

Different yeast strains were cultivated in the LAMBDA MINIFOR 0.4L Fermentor to study the metabolic cylce and pathway. 

J. Feltham, S. Xi, S. Murray, M. Wouters, J. Urdiain-Arraiza, C. George, A. Townley, E. Roberts, R. Fisher, S. Liberatori, S. Mohammed, B. Kessler & J. Mellor. (2020). Transcriptional changes are regulated by metabolic pathway dynamics but decoupled from protein levels. bioRxiv 833921.

https://doi.org/10.1101/833921

LAMBDA MINIFOR Bioreactor is used as a Rumen membrane bioreactor to produce volatile fatty acids (VFA) from crop residues (lignocellulosic biomass) by mimicking the digestive system of ruminant animals.

Nguyen, A.Q., Nguyen, L.N., Abu Hasan Johir, M., Ngo, H-H., Chaves, A.V. & Nghiem, L.D. (2020) Derivation of volatile fatty acid from crop residues digestion using a rumen membrane bioreactor: a feasibility study. Bioresource Technology 2020.

https://doi.org/10.1016/j.biortech.2020.123571

Enzymatic hydrolysis experiments were carried out in the lab-scale LAMBDA MINIFOR stirred-batch bioreactor. The pretreated vine-shoot waste was delignified with sodium chlorite for lignin removal and then enzymatically hydrolyzed using new types of enzymes (cellulase from Trichoderma reesei and b-glucosidase).

Eniko Kovacs, Daniela Alexandra Scurtu, Lacrimioara Senila, Oana Cadar, Diana Elena Dumitras & Cecilia Roman (2020). Green Protocols for the Isolation of Carbohydrates from Vineyard Vine-Shoot Waste. Analytical Letters.

https://doi.org/10.1080/00032719.2020.1721001

LAMBDA MINIFOR bioreactor used to produce Itaconic Acid biotechnologically by Aspergillus terreus fungal strain from glucose

Nemestóthy, N., Komáromy, P., Bakonyi, P. et al. (2020). Carbohydrate to Itaconic Acid Conversion by Aspergillus terreus and the Evaluation of Process Monitoring Based on the Measurement of CO2 Waste and Biomass. Valorization 2020.

https://doi.org/10.1007/s12649-019-00729-3

2019: 

LAMBDA MINIFOR bioreactor used in turbidostat experiments with recombinant cells in continuous culture operation mode

Pasotti, L., Bellato, M., Politi, N., Casanova, M., ucca, S., Gabriella, M., De Angelis, C. & Magni, P. (2019). A synthetic close-loop controller circuit for the regulation of an extracellular molecule by engineered bacteria. IEEE Trans Biomed Circuits Syst. 2019 Feb; 13(1):248-258.

https://doi.org/10.1109/TBCAS.2018.2883350

pH optimization for the aerobical production of itaconic acid catalyzed by Aspergillus terreus in a LAMBDA MINIFOR bioreactor: Batch, working volume 1.8 L, medium with 120 g/L glucose substrate, 37 °C, pH 3 - pH 2.5 - pH 4 - pH 3 - pH 2.5, stirrer 2 Hz, aeration 1.5 L (STP)/min.

Komáromy, K., Bakonyi, P., Kucska, A., Tóth, G., Gubicza, L., Bélafi-Bakó, K. & Nemestóthy, N. (2019). Optimized pH and Its Control Strategy Lead to Enhanced Itaconic Acid Fermentation by Aspergillus terreus on Glucose Substrate. Fermentation 2019, 5(2), 31

https://doi.org/10.3390/fermentation5020031

Lab-Scale Production of Rhamnolipid by Pseudomonas Aeruginosa A3 using LAMBDA MINIFOR Benchtop Bioreactor.

Faqri, A. F., Hayder, N.H. & Hashim, A.J. (2019). Lab-scale production of Rhamnolipid by Pseudomonas Aeruginosa A3 and study its synergistic effect with certain antibiotics against some pathogenic bacteria. Iraqi Journal of Agricultural Sciences –2019:50(5):1290-1301.

https://doi.org/10.13140/RG.2.2.10802.35520 

2018: 

A large-scale pro-siRNA production method was developed in a LAMBDA MINIFOR bioreactor for high yield production of pro-siRNA 

Kaur, G., Cheung, H. C., Xu, W., Wong, J. V., Chan, F. F., Li, Y., McReynolds, L. & Huang, L. (2018). Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli. Biotechnology and Bioengineering, 115(9), 2280-2291.

https://doi.org/10.1002/bit.26740

LAMBDA MINIFOR lab scale bioreactor used for production of bioethanol from lignocellulosic biodegradable municipal solid waste (BMSW) under optimized conditions

Hayder, N. H., Flayeh, H. M., & Ahmed, A. W. (2018). Optimization of bioethanol production from biodegradable municipal solid waste using response surface methodology (RSM). Journal of Engineering and Sustainable Development, 22(1), 47-64. 

https://www.iasj.net/iasj/download/28dcbea4ab5f5ba8 (2024 Feb. 05)

2017:

Comparison of the experimental and theoretical production of biogas. The LAMBDA MINIFOR bioreactor filled with 2 litres of inoculum was incubated anaerobically at 35 °C for 1 month. 

El Asri, O., & Afilal, M. E. (2018). Comparison of the experimental and theoretical production of biogas by monosaccharides, disaccharides, and amino acids. International Journal of Environmental Science and Technology, 15(9), 1957-1966.

https://doi.org/10.1007/s13762-017-1570-1

Study of the metabolism of isolated lamb’s lettuce cells (Valerianella locusta (L). Laterr.) upon sugar starvation under O2 stress conditions using 13C [U-13C6] labelled glucose in a LAMBDA MINIFOR bench-top laboratory bioreactor (dark, 250 ml, pH 5.8, 18 °C, airation 10 L/h)

Victor, B. M. M., Ampofo-Asiama, J., Hertog, M., Geeraerd, A. H., & Nicolai, B. M. (2017). Metabolic profiling reveals a coordinated response of isolated lamb's (Valerianella locusta, L.) lettuce cells to sugar starvation and low oxygen stress. Postharvest Biology and Technology, 126, 23-33. 

https://doi.org/10.1016/j.postharvbio.2016.12.004

LAMBDA MINIFOR fermenters used as continuous anaerobic flow stirred digesters (CSTR) for anaerobic digestion of organic solid waste

Nakasima-López, M., Taboada-González, P., Aguilar-Virgen, Q., & Velázquez-Limón, N. (2017). Adaptación de inóculos durante el arranque de la digestión anaerobia con residuos sólidos orgánicos. Información tecnológica, 28(1), 199-208.

https://dx.doi.org/10.4067/S0718-07642017000100020

The effect of different temperatures on sugar starvation in cells isolated from fresh leafy vegetables was studied in LAMBDA MINIFOR bioreactor

Mbong, V. B. M., Ampofo-Asiama, J., Hertog, M. L., Geeraerd, A. H., & Nicolai, B. M. (2017). The effect of temperature on the metabolic response of lamb’s lettuce (Valerianella locusta,(L), Laterr.) cells to sugar starvation. Postharvest Biology and Technology, 125, 1-12.

https://doi.org/10.1016/j.postharvbio.2016.10.013

LAMBDA MINIFOR bioreactor for the production of CB.Hep-1 mAb using mouse hybridoma cell culture in protein-free media

Valdés, R., Aragón, H., González, M., Hernández, D., Geada, D., Goitizolo, D., Ferro, W., Pérez, A., García, J., Masforrol, Y, Aguilar, P., Márquez, G., LaO, M., González, T., Calvo, Y., Hernández, A.,  Menéndez, G. & Tamayo, A. (2017). Mouse Hybridoma Cell Culture in a Protein-Free Medium Using a Bio-Mimicking Fish-Tail Disc Stirred Bioreactor. BioProcessing Journal, 16(1).

https://doi.org/10.12665/J161.Valdes

2016:

Robust cellulosic ethanol production from sugarcane bagasse with Saccharomyces cerevisiae ATCC 20602 in LAMBDA MINIFOR laboratory bioreactor under aerobic and anaerobic conditions with controlled redox potential measurement

Jabasingh, S. A., Lalith, D., Prabhu, M. A., Yimam, A., & Zewdu, T. (2016). Catalytic conversion of sugarcane bagasse to cellulosic ethanol: TiO2 coupled nanocellulose as an effective hydrolysis enhancer. Carbohydrate polymers, 136, 700-709.

https://doi.org/10.1016/j.carbpol.2015.09.098

2015:

Bioreactor system LAMBDA MINIFOR as an artificial mouth system for the growth of biofilms

LA BOCA ARTIFICIAL DE DENTAID UNA REVOLUCIÓN EN INVESTIGACIÓN BUCODENTAL
DENTAID EXPERTISE, PUBLICACIÓN PARA PROFESIONALES DE LA ODONTOLOGÍA, NÚM. 18.

https://aprenderly.com/doc/3463742/la-boca-artificial-de-dentaid-una-revolución-en-investiga…?page=5 (2024 Feb. 05)

S. pyogenes Cas9 protein was expressed in E. coli using a computer-controlled LAMBDA MINIFOR 3L bioreactor in batch mode followed by exponential feeding

Ménoret, S., De Cian, A., Tesson, L., Remy, S., Usal, C., Boulé, J. B., Boix, C., Fontanière, S., Crénéguy, A., Nguyen, T.H., Brusselle, L., Thinard, R., Gauguier, D., Concordet, J.-P., Cherifi, Y., Fraichard, A., Giovannangeli, C. & Anegon, I. (2015). Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins. Scientific reports, 5(1), 14410. 

https://doi.org/10.1038/srep14410

Fermentation of engineered microorganism in laboratory scale bioreactor LAMBDA MINIFOR for efficient conversion of lactose-to-ethanol

Pasotti, L., Zucca, S., Casanova, M., Massaiu, I., Mazzini, G., Micoli, G., Calvio, C., Cusella de Angelis, M.G. & Magni, P. (2015, August). Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology. In 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) (pp. 953-956). IEEE. 

https://doi.org/10.1109/EMBC.2015.7318521

Six-species flow cell biofilm model was developed by culturing bacteria in LAMBDA MINIFOR bioreactor to evaluate the biofilm development under flow and shear conditions

Salli, K. M., & Ouwehand, A. C. (2015). The use of in vitro model systems to study dental biofilms associated with caries: a short review. Journal of oral microbiology, 7(1), 26149. 

https://doi.org/10.3402/jom.v7.26149 

Quantification of ribosomal proteins (RPs) from Yeast cells cultured in LAMBDA MINIFOR bioreactor and mouse embryonic stem cells (ESC) to study the core RPs stoichiometry

Slavov, N., Semrau, S., Airoldi, E., Budnik, B., & van Oudenaarden, A. (2015). Differential stoichiometry among core ribosomal proteins. Cell reports, 13(5), 865-873. 

https://doi.org/10.1016/j.celrep.2015.09.056 

Harvard University, USA; Broad Institute of MIT and Harvard, USA and Hubrecht Institute, Netherlands.

2014:

Cultivation of microalgae (Chlorella vulgaris Beyerinck) in laboratory bioreactor LAMBDA MINIFOR

Heitur, H. (2014). Mikrovetika Chlorella vulgaris Beyerincki kasvatamine CO2 sidumise eesmärgil (Master's thesis). 

https://hdl.handle.net/10492/1842 (2024 Feb. 05)

Eesti Maaülikool (Estonian University of Life Sciences), Estonia.

Growing yeast cultures (DBY12007) in the LAMBDA MINIFOR fermenter at steady state to study the aerobic glycolysis and energy flux

Slavov, N., Budnik, B. A., Schwab, D., Airoldi, E. M., & van Oudenaarden, A. (2014). Constant growth rate can be supported by decreasing energy flux and increasing aerobic glycolysis. Cell reports, 7(3), 705-714. 

https://doi.org/10.1016/j.celrep.2014.03.057 

Massachusetts Institute of Technology, USA; Harvard University, USA; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, Netherlands and Princeton University, USA.

Selective and non-selective batch fermentation of date extract using Saccharomyces cerevisiae (commercial strain used in bakeries (wild strain), glucose selective strains ATCC 36858 and ATCC 36859) studied in LAMBDA MINIFOR fermentor

Putra, M. D., Abasaeed, A. E., Zeinelabdeen, M. A., Gaily, M. H., & Sulieman, A. K. (2014, April). Selective fermentation of pitted dates by S. cerevisiae for the production of concentrated fructose syrups and ethanol. In Journal of Physics: Conference Series (Vol. 495, No. 1, p. 012034). IOP Publishing. 

https://doi.org/10.1088/1742-6596/495/1/012034 

King Saud University, Chemical Engineering Department, Saudi Arabia 

The metabolic stress response of tomato cell culture (Lycopersicum esculentum) to low oxygen studied using LAMBDA MINIFOR Bioreactor

Ampofo‐Asiama, J., Baiye, V. M. M., Hertog, M. L. A. T. M., Waelkens, E., Geeraerd, A. H., & Nicolai, B. M. (2014). The metabolic response of cultured tomato cells to low oxygen stress. Plant Biology, 16(3), 594-606. 

https://doi.org/10.1111/plb.12094 

KU Leuven, Belgium; Flanders Centre of Postharvest Technology (VCBT), Leuven, Belgium.

LAMBDA MINIFOR bioreactor to grow the oral bacteria (Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis) under planktonic conditions

Blanc, V., Isabal, S., Sanchez, M. C., Llama‐Palacios, A., Herrera, D., Sanz, M., & León, R. (2014). Characterization and application of a flow system for in vitro multispecies oral biofilm formation. Journal of Periodontal Research, 49(3), 323-332. 

https://doi.org/10.1111/jre.12110 

DENTAID S. L., Cerdanyola del Vallès, Spain; ETEP Research Group, University Complutense of Madrid, Spain. 

LAMBDA MINIFOR Bioreactor used for recombinant protein (Chemokines) expression in E. coli 

Kramp, B. (2014). Establishing the interaction between the CC chemokine ligand 5 and the receptors CCR1 and CCR (Doctoral dissertation, Aachen, Techn. Hochsch., Diss., 2013). 

https://core.ac.uk/download/pdf/36589112.pdf (2024 Feb. 05)

RWTH Aachen, Germany.

Medium optimization and growth kinetics of S. marcescens were studied in a benchtop bioreactor LAMBDA MINIFOR 7L Advanced Kit: batch cultivations at 30 °C, 3 L/min aeration, 4 Hz stirring, pH 6, polypropylene glycol as antifoam.

Mohammed, S. J. (2014). Optimization Of medium Composition for increasing prodigiosin production by local isolate of Serratia marcescens. Doctoral thesis, Al-Khwarizmi College of engineering, Department of Biochemical engineering, University of Baghdad.
https://doi.org/10.13140/RG.2.2.18638.7968 

2013: 

Development of biofilm model (S. oralis, A. naeslundii, V. parvula, F. nucleatum, A. actinomycetemcomitans and P. gingivalis) in continuous flow in a LAMBDA MINIFOR 0.4L bioreactor.

Soto, I. S. (2013). Desarrollo del modelo de boca artificial en flujo continuo en el biorreactor Lambda Minifor. Universidad Complutense de Madrid Master en Ciencias Odontológicas.

https://docta.ucm.es/rest/api/core/bitstreams/39d8318b-a49b-4cb3-a164-e4faab18f52b/content (2024 Feb. 05)

Recombinant expression of the Met-CCL5, protease resistant CXCL12 (S4V) and F1-CX3CL1 in E. coli using LAMBDA MINIFOR fermenter/bioreactor to study their role in Cardiovascular disease (CVD)

Projahn, D. (2013). Generation, function and therapeutic application of chemotactic cytokines in cardiovascular diseases (Doctoral dissertation, Aachen, Techn. Hochsch., Diss., 2013). 

https://publications.rwth-aachen.de/record/229207/files/4840.pdf (2024 Feb. 05)

RWTH Aachen, Germany.

Expression of Caf1 protein using Escherichia coli strain in LAMBDA MINIFOR fermentor to study mammalian cell adhesion, shape and number of focal adhesion

Machado Roque, A. I. (2013). Protein scaffolds for cell culture (Doctoral dissertation, Newcastle University). 

URI: https://hdl.handle.net/10443/1843 (2024 Feb. 05)

Newcastle University, UK.

Controlled growth of Staphylococcus aureus under various concentrations of BAC (benzalkonium chloride) in LAMBDA MINIFOR fermentor

Cervinkova, D., Babak, V., Marosevic, D., Kubikova, I., & Jaglic, Z. (2013). The role of the qacA gene in mediating resistance to quaternary ammonium compounds. Microbial Drug Resistance, 19(3), 160-167. 

https://doi.org/10.1089/mdr.2012.0154

Veterinary Research Institute, Brno, Czech Republic.

2012:

Effective production of Biobutanol from agricultural waste (giant hogweed, hay) using LAMBDA MINIFOR bench-top laboratory fermenter

Mezule, L., Tihomirova, K., Nescerecka, A., & Juhna, T. (2012). Biobutanol production from agricultural waste: A simple approach for pre-treatment and hydrolysis. Latvian Journal of Chemistry, 51(4), 407. 

https://doi.org/10.2478/v10161-012-0028-5 

2011:

Bioethanol production using Yeast (S. cerevisiae) in LAMBDA MINIFOR fermenter

Burešová, I., & Hřivna, L. (2011). Effect of wheat gluten proteins on bioethanol yield from grain. Applied Energy, 88(4), 1205-1210. 

https://doi.org/10.1016/j.apenergy.2010.10.036 

2010:

Anaerobic fermentation of the glucose component in dates extract by yeast Saccharomyces cerevisiae

Gaily, M. H., Elhassan, B. M., Abasaeed, A. E., & Al-Zahrani, S. M. (2010). A direct process for the production of high fructose syrups from dates extracts. International Journal of Food Engineering, 6(3). 

https://doi.org/10.2202/1556-3758.1879 

King Saud University, Saudi Arabia; University of Khartoum, Sudan 

Study of the potential of tree tobacco stems (Nicotiana Glauca r. Grah.) as a bioethanol feedstock with the LAMBDA MINIFOR fermenter

Sánchez, F., Curt, M. D., Barreiro, M., Fernández, J., Agüera, J. M., Uceda, M., & Zaragoza, G. (2010). TREE TOBACCO (NICOTIANA GLAUCA R. GRAH.) STEMS AS A BIOETHANOL FEEDSTOCK. 18th European Biomass Conference and Exhibition, 3-7 May 2010, Lyon, France. 

https://www.researchgate.net/profile/Francisco-Sanchez-13/publication/290439533_Tree_Tobacco_Nicotiana_glauca_RGrah_Stems_as_a_Bioethanol_Feedstock/links/56fa3d4608ae81582bf44ca4/Tree-Tobacco-Nicotiana-glauca-RGrah-Stems-as-a-Bioethanol-Feedstock.pdf (2024 Feb. 05)

Dpt. Producción Vegetal: Botánica y Protección Vegetal. Universidad Politécnica de Madrid (UPM), Madrid, Spain

2009:

Determination of the alcoholigenous potential of non-cellulosic carbohydrates from prickly pear cladodes by fermentation with the yeast Saccharomyces cerevisiae (commercial strains)

Sánchez, F., Curt, M. D., Fernández, J., Agüera, J. M., Uceda, M., & Zaragoza, G. (2009). Bioethanol production from prickly pear (Opuntia ficus-indica (L.) Mill.) cladodes. In Proc. 17th European Biomass Conference. Pub. ETA-Florence Renewable Energies & WIP-Renewable Energies. ISBN (pp. 978-88). 

https://www.researchgate.net/profile/Francisco-Sanchez-13/publication/290439312_Bioethanol_Production_from_Prickly_Pear_Opuntia_ficus-indica_L_Mill_Cladodes/links/56fa3edd08ae38d710a31fc1/Bioethanol-Production-from-Prickly-Pear-Opuntia-ficus-indica-L-Mill-Cladodes.pdf (2024 Feb. 05)

Dpt. Producción Vegetal: Botánica y Protección Vegetal. Universidad Politécnica de Madrid (UPM), Madrid, Spain

2007:

Anaerobic expression using the LAMBDA MINIFOR

Park, M. O., Mizutani, T., & Jones, P. R. (2007). Glyceraldehyde-3-phosphate ferredoxin oxidoreductase from Methanococcus maripaludis. Journal of bacteriology, 189(20), 7281-7289. 

https://doi.org/10.1128/jb.00828-07 

Research and Development Division, Fujirebio Incorporated, Japan.

2006:

Systems for High-Density Hybridoma Growth and High-yield mAb production in cell culture: Bench-top stirred tank bioreactors, 1-5 L (MINIFOR - LAMBDA Laboratory Instruments)

Howard, G.C., & Kaser, M.R. (Eds.). (2006). Making and Using Antibodies: A Practical Handbook (1st ed.). CRC Press.

https://doi.org/10.1201/9781420005196   

2005:

pH and temperature continuously recorded with the LAMBDA MINIFOR and SIAM software

Chaignon, P., Cortial, S., Guerineau, V., Adeline, M. T., Giannotti, C., Fan, G., & Ouazzani, J. (2005). Photochemical Reactivity of Trifluoromethyl Aromatic Amines: The Example of 3, 5‐diamino‐trifluoromethyl‐benzene (3, 5‐DABTF). Photochemistry and photobiology, 81(6), 1539-1543. 

https://doi.org/10.1562/2005-08-03-RA-637 

Institut de Chimie des Substances Naturelles, C.N.R.S, France.

2003:

Bioreactors - An overview of the innovations implemented in MINIFOR bioreactors

Lehky, P. (2003) Bioreactors - New Solutions for Old Problems. International Congress on Bioreactor Technology, Tampere, Finland. 

www.bioreactors.eu/files/bioreactor/minifor-bioreactors-article.pdf (2024 Feb. 05)

Do you sell/ship to the USA?

Yes, we do supply our instruments directly with door-to-door delivery option by the parcel services to the USA.

What is the availability of the product?

We have the instruments in stock. We would just have to configure the instruments according to your requirements and perform quality control before shipping.

Is there a warranty?

We offer a 2 year warranty for MINIFOR fermentor / bioreactor and 5 year warranty for the PRECIFLOW & MULTIFLOW peristaltic pumps.

Does this fermentor work on both mammalian cells and yeast cells?

Yes, MINIFOR fermentor and bioreactor can be used for mammalian and yeast cell cultures (More information at www.fermentor.net/applications).

Is there flexibility in the top plate to add or remove probes?

Yes, MINIFOR has free ports in the headspace for the additional probes (sensors). Multiple ports and other effective solutions in the fermentation glass vess make the MINIFOR configuration equivalent to 16 to 22 classical ports (it is possible to increase the number of ports – custom made solution)

Is the equipment suitable for use in pure / mixed culture?

Yes, MINIFOR is suitable for pure as well as mixed culture. The stirrer is strong and can easily be adapted according to the types of cultures and working volumes.

Why is MINIFOR perfectly suitable for parallel processes?

Each unit stays independent as it is equipped with a control panel and display and at a single glance shows the parameter values. All parameters are regulated locally inside each fermenter-bioreactor unit.

This allows fast and precise parameter regulation and never having to worry about leaving a vessel unattended. Further advantage is that in case there are problems with one unit, the other units will still keep running.

How important is the slowdown in parameter regulation while running 12 bioreactors in parallel?

An important aspect to consider – which, however, does not play a role in the LAMBDA MINIFOR parallel system because each MINIFOR fermenter comes with its proper regulation unit that measures and controls all parameters locally. As a consequence the quality of the measurement and regulation is not affected by long transmission times and dead times in regulation.

How much space is required for the MINIFOR unit?

Footprint: approximately a sheet of paper
Dimensions: 22 cm x 38 cm x 40 cm (W x H x D)